🚀 go-pugleaf

I am using the toluene based low viscosity mounting medium "Cytoseal 60"
fl.oz. Works great.

Steffen Dietzel wrote:
>
> Anda,
>
> wheras I never had problems with overnight fixation of cells in 1.6 %
> formaldehyde, I did have problems with the nailpolish: I have tried several
> ones and they all killed the GFP before the slide was even on the scope. I have
> heard rumors of nailpolish that does not harm GFP, but was never able to hunt
> one down, therefore PLEASE:
> Could you let us know the exact brand name, product number or whatever is
> usefull to find the nailpolish you are using?
>
> Thanks!  Steffen
>
> --- Anda Cornea <corneaan@OHSU.EDU> schrieb:
> >
> > >>> "Goodhouse, Joseph" Long
> > For fixation employ low percentage paraformaldehyde for the shortest periods
> > of fixation as possible( 5- 10 minutes for cells).  Samples should be
> > mounted in glycerol based mounting medium.  I have found 90% buffered
> > glycerol to be best.  Avoid using nail polish as sealant of coverslips.  In
> > a short time this will wipe out your GFP signal.
> >
> > We routinely use 4%paraformaldehyde, 30minutes fixation, for cells expressing
> > a GFP-membrane receptor chimera and seal slides with nailpolish.  We see no
> > loss of intensity when compared with live cells expressing the same
> > construct, not even after a few months storage.
> >
>
> =====
> Dr. Steffen Dietzel
> Dept. of Cell and Structural Biology
> University of Illinois at Urbana-Champaign
> 601 S. Goodwin Ave.
> Urbana, IL 61801
> Phone: +1/217/333-8372  Fax: +1/217/244-1648
> e-mail: dietzels@yahoo.de  Web:  http://www.life.uiuc.edu/belmont/dietzel
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Edward Monosov, Ph.D.
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