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Article #157Re: Leica TCN SP laser power
From: Dr. Sudipta Maiti
Date: Mon, 13 Mar 2000 00:00
Date: Mon, 13 Mar 2000 00:00
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This is a multi-part message in MIME format. --------------23FA19AE4B1871B6F616158A Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit When you put the power meter on the stage, there would (presumably) be an air gap between the objective and the power meter. Would that not prevent the higher angle rays from coming out of a high NA immersion objective (due to total internal reflection)? I guess if the requirements are not stringent then measuring the power at the back aperture (putting in a hole the size of the back aperture instead of the lens) and assuming a reasonable transmittance by the objective (say 0.9 or 0.95 for 514 nm) would be a closer approximation to the actual laser power at the sample. Sudipta --------------23FA19AE4B1871B6F616158A Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Sudipta Maiti Content-Disposition: attachment; filename="vcard.vcf" begin: vcard fn: Sudipta Maiti n: Maiti;Sudipta org: Tata Institute of Fundamental Research adr: Dept. of Chemical Sciences, Tata Institute of Fundamental Research;;Homi Bhabha Road, Colaba;Mumbai;Maharashtra;400 005;India email;internet: maiti@tifr.res.in title: Fellow tel;work: 91-22-215-2971 ext.2258/2526 tel;fax: 91-22-215-2110 tel;home: 91-22-218-4980 x-mozilla-cpt: ;0 x-mozilla-html: FALSE version: 2.1 end: vcard --------------23FA19AE4B1871B6F616158A--
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 114d69,a87a0a80e0ec3844 X-Google-Attributes: gid114d69,public From: "Dr. Sudipta Maiti" <maiti@TIFR.RES.IN> Subject: Re: Leica TCN SP laser power Date: 2000/03/13 Message-ID: <38CC6446.9749C7C8@tifr.res.in> X-Deja-AN: 596697009 Sender: Confocal Microscopy List <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU> References: <852568A1.0010FA07.00@EPAHUB2.RTP.EPA.GOV> Content-Type: multipart/mixed; boundary="------------23FA19AE4B1871B6F616158A" X-Trace: 12 Mar 2000 22:42:18 -0500, 127.0.0.1 Organization: Dept. of Chemical Sciences, Tata Institute of Fundamental Research X-Gateway-From: add_from_switch_to_drobot MIME-Version: 1.0 Reply-To: Confocal Microscopy List <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU> Newsgroups: bit.listserv.confocal