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Article #231does someone know concanavalin A
From: Feng L
Date: Fri, 23 Apr 2004 03:18
Date: Fri, 23 Apr 2004 03:18
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I recently read a paper (JBC, 1975, pp1513-1524) talking about Concanavalin A. It said both Mn2+ and Ca 2+ metal ions appeared to be required for saccharide binding to occur.My question is if I want that glucose binds ConA, do I have to use buffer with Ca2+ and Mn2+ in the solution in order to get better binding result??. Could someone give me a answer? thanks.
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 114d69,f642fcc8ba82b749,start X-Google-Attributes: gid114d69,public X-Google-ArrivalTime: 2004-04-22 20:33:35 PST Path: arc1-ca.pugleaf.net!archiver1.google.com!news1.google.com!news.glorb.com!pln-e!lotsanews.com!news-hog.berkeley.edu!ucberkeley!enews.sgi.com!news.tamu.edu!not-for-mail From: "Feng L" <fengL76@hotmail.com> Newsgroups: bit.listserv.confocal Subject: does someone know concanavalin A Date: Thu, 22 Apr 2004 22:18:02 -0500 Organization: Texas A&M University, College Station, Texas Lines: 7 Message-ID: <c6a1tf$3ep$1@news.tamu.edu> NNTP-Posting-Host: rich-dhcp-0440.tamu.edu NNTP-Posting-Date: 23 Apr 2004 03:18:07 GMT X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 6.00.2800.1409 X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1409