Re: GFP and greening tissue

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#51

From: Carol Heckman
Date: Fri, 10 Mar 2000 00:00

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Steve --
A lot of fluorescence in the yellow-green wavelengths is introduced by the
formaldehyde reacting with tissue components.  You can fix for just 10
minutes and then store tissues indefinitely in phosphate-buffered saline at
4 degrees C.  I haven't noticed any deterioration in the strength of the
GFP fluorescence under these conditions, and I have viewed samples up to
several months later.
Carol Heckman

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From: Carol Heckman <heckman@BGNET.BGSU.EDU>
Subject: Re: GFP  and greening tissue
Date: 2000/03/10
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