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Article #63pH sensitive fluorescent dyes
From: Ferbs
Date: Fri, 28 Oct 2005 11:15
Date: Fri, 28 Oct 2005 11:15
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1736 bytes
Dear all, I am looking to find a pH sensitive fluorescent dye that exhibits a greater fluorescence in low pH situations (as the concentration of H ions increases I want it to be brighter). This is the opposite of the response to pH that most dyes show (e.g. fluorescein). Probes have a dye called lysosensor yellow/blue I think that does what I'm looking for, however it is excited in the UV region and I really want one that can be excited on most confocals (e.g. 488 nm or 568 nm). Does anyone have any suggestion of a suitable dye and where I get some? Ta Andy B.
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