Cy5

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#7740

From: Charles J. Butterick
Date: Tue, 30 May 1995 00:00

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Greetings,
       A number of people seem to be using Cy5.  An investigator here wants
to use it in a dual labeling experiment.  My concern is in the detection of
the emission.  Our Olympus confocal uses a Hamamatsu R928 PMT with a
spectral response curve that suggests 30-50% detection at the Cy5 emission
peak of 680 nm.  Has anyone else had to deal with this problem?  Is it a
problem?  What kind of filter setups are being used with Cy5?  Thanks for
any help.



  Charles J. Butterick  (Chuck)
  Electron Microscopy Center
  Department of Cell Biology
       and Biochemistry
  Texas Tech University Health
       Sciences Center
  3601 4th Street
  Lubbock, Texas  79430

  vox    (806) 743-1633
  fax    (806) 743-1219
  email  emccjb@lubb.ttuhsc.edu or
         chuck@micron1.lubb.ttuhsc.edu

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From: "Charles J. Butterick" <emccjb@TTUHSC.EDU>
Subject: Cy5
Date: 1995/05/30
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