Endogenous biotin

Article View: bit.listserv.confocal

Article #7792

Back to bit.listserv.confocal Previous Next

#7792

From: Natalie Bilyk
Date: Fri, 19 May 1995 00:00

13 lines
992 bytes

Hi everyone,
We are immunostaining for
macrophages and dendritic cells.
and have encountered what appears to be endogenous biotin as avidin
conjugates alone seemed to stain tissue.  Has anyone encountered this
problem before and are they aware of
any techniques that block endogenous biotin in tissue prior to staining?
Look forward to your replies.

Natalia Bilyk
Dept Anatomy and Human Biology
University of Western Australia
 email nbilyk@uniwa.uwa.edu.au

Technical Details

Message-ID: <Pine.ULT.3.90.950519120646.23254A-100000@uniwa.uwa.edu.au>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 114d69,56f9287ad41758a3
X-Google-Attributes: gid114d69,public
From: Natalie Bilyk <nbilyk@UNIWA.UWA.EDU.AU>
Subject: Endogenous biotin
Date: 1995/05/19
Message-ID: <Pine.ULT.3.90.950519120646.23254A-100000@uniwa.uwa.edu.au>
X-Deja-AN: 102893577
sender: Confocal Microscopy List <CONFOCAL@UBVM.CC.BUFFALO.EDU>
comments: Gated by NETNEWS@AUVM.AMERICAN.EDU
content-type: TEXT/PLAIN; charset=US-ASCII
mime-version: 1.0
newsgroups: bit.listserv.confocal