Dichroic mirrors...help

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#7803

From: M.J. Tobin
Date: Tue, 23 May 1995 00:00

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Hello everyone
I am currently using a custom built confocal microscope which employs a
polarizing beamsplitter and a wave retarder in place of a dichroic mirror,
allowing reflection and fluorescence images to be collected simultaneously.
However I am planning to modify the instrument to perform fluorescence
polarization imaging and the optical layout of the microscope means that I
will need a dichroic mirror that transmits short wavelengths (excitation) and
reflects longer wavelengths (fluorescence).  As far as I can tell the
dichroics supplied with conventional microscopes work the other way around.
Does anybody out there use, or know of a manufacturer who supplies mirrors that
will work in this way?
I noticed in a paper by Shingo Kashima in the latest issue of "Scanning", the
optical layout shows a dichroic mirror working in just the way I require but
can find no reference to it in the text.  (Vol 17,2  1995  p67 fig 1)

If you can help me, please email me direct to save filling everyone elses
mailboxes.

Thanks in advance!

Mark Tobin
Biological Spectroscopy Group
Daresbury Laboratory
Daresbury
Warrington
WA4 4AD      UK

email    m.tobin@dl.ac.uk

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From: "M.J. Tobin" <M.J.Tobin@DL.AC.UK>
Subject: Dichroic mirrors...help
Date: 1995/05/23
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