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Article #7807

Re: Endogenous biotin

#7807
From: Karl Bellve
Date: Tue, 23 May 1995 00:00
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Hi Margaret (Margaret Fransen), in <9505231446.AA01929@umabnet.ab.umd.edu> on Ma
y 23 you said

> Regarding possible endogenous ligands for streptavidin, there was a report
> in 1994 (or 93) that streptavidin bound to integrins.  Perhaps that could
> account for some "non-specific" labelling with biotin/avidin techniques.
> Margaret Fransen, Ph.D.
> Dept. of Anatomy & Cell Biology
> SUNY Health Science Center @ Syracuse
> 750 E. Adams St. Syracuse NY 13210
> Tel: 315 464 8525
> Fax: 315 464 8535
> E-mail: FransenM@vax.cs.hscsyr.edu
>
>

This is TRUE, also plain avidin has alot of sugar groups on its surface.
I use Neutralite Avidin from Molecular Probes. It has its sugar groups
striped.



Karl Bellve                             LLLL  L     L LLLLL  LLLL    LLLL
University of Maryland at Baltimore    L    L LL   LL   L   L    L  L    L
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From: Karl Bellve <KBellve@UMABNET.AB.UMD.EDU>
Subject: Re: Endogenous biotin
Date: 1995/05/23
Message-ID: <20b571d9.31fd5-KBellve@wade1.ab.umd.edu>
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              <fransenm@vax.cs.hscsyr.edu>) (at tue, 23 may 1995 10: 15:23 -0500)
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