Re: Visible DNA Stain

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#97

From: Jurek Dobrucki
Date: Fri, 17 Mar 2000 00:00

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> We use DAPI and the nucleoli with all their RNA remain unstained, while the
> nuclear DNA stains.  However, you need a UV laser.  Nina Allen>

A UV laser is not absolutely necessary to excite DAPI
fluorescence. DAPI can also be excited using a 457 line which is
available on our air-cooled 100mW Ar+ laser. Unortunately this line
is not available in the case of smaller 25 mW Ar+ lasers.

Jurek Dobrucki


=======================================================
J.Dobrucki
Laboratory of Confocal Microscopy and Image Analysis
Department of Biophysics, Jagiellonian University
Al. Mickiewicza 3
31-120 Krakow, Poland
    tel. (48-12) 634-14-42, ex. 268, fax. (48-12) 633-69-07
    Internet: dobrucki@mol.uj.edu.pl
    WEB:      http://helios.mol.uj.edu.pl/
=======================================================

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From: Jurek Dobrucki <DOBRUCKI@MOL.MOL.UJ.EDU.PL>
Subject: Re: Visible DNA Stain
Date: 2000/03/17
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