Help with beam splitters for triple CFP/YFP/RFP imaging??

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1 total messages Started by Sean Megason Fri, 09 Jul 2004 23:28

#234

Author: Sean Megason
Date: Fri, 09 Jul 2004 23:28

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I want to do triple imaging of ECFP, EYFP, and DsRed on a Leica TCS SL
confocal. I would ideally like to capture all 3 channels
simultaneously (for speed) by exciting simultaneously with 458, 514,
and 543 lines. This requires a triple dichroic beam splitter. Does
anyone know of the existence of a 458/514/543 beam splitter?

If this isn't possible, I'd like to capture the ECFP and DsRed in one
pass and capture the EYFP in a second pass. This would require a
458/543 double dichroic beam splitter for doing the ECFP/DsRed. I know
these exist because I've seen them on Zeiss confocals but I (and my
Leica rep) can't figure out what manufacturer makes a 458/543
dichroic. Anyone know??

Sean Megason

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