🚀 go-pugleaf

In article <YMIWA.92Oct15203724@virus4.virus.kyoto-u.ac.jp> ymiwa@virus.kyoto-u.ac.jp (Yoshihiro Miwa) writes:
> $B5~ETBg3X%&%$%k%98&5f=j$N(B $B;0NX$G$9!#(B

$B$I$&$b!";O$a$^$7$F!#CS!wM}8&$G$9!#%U%)%m!<CY$l$F$9$_$^$;$s!#(B
$B>!<j$J$,$i9T4V$r>/$75M$a$5$;$F$$$?$@$-$^$9!#$=$l$H855-;v$+$i>/$7;~4V$,(B
$BN)$C$F$$$k$N$G!"0zMQ$rD9$/$7$^$9!#(B

>$B$J$K$d$i(Btelomere$B$K4X$7$F5DO@$,$J$5$l$F$$$k$h$&$G$9$M!#(B
>$B#2!"#3G/A0$K6=L#$r$b$C$FJY6/$7$?$3$H$,$"$k$N$G!"$=$N$3$m$N5-21$r(B
>$B$b$H$KJ,$+$kHO0O$N$3$H$r=q$$$F$_$^$9!#(B

$B$O$$!#$h$/$4B8CN$NJ}$N@bL@$,0lHV$$$$$G$9$h$M!#(B

>telomere$B$NG[Ns$K$D$$$F$O!"4{$KEj9F$5$l$F$$$?$h$&$K(BTTGGGG,TTTTGGGG,TTAGGG
>$B$H$$$C$?(BG-rich$B$JG[Ns$K$J$C$F$$$^$9!#$H$/$K$h$/8&5f$5$l$F$$$k$N$,(BTTGGGG$B$N(B
>$BG[Ns$r$b$D(BTetrahymena$B$J$N$G$9$,!"$3$N@8J*$O@83h4D$N$J$+$G!"Bg3K$NCf$K!"(B
>$B$?$/$5$s$NC;$$@w?'BN$r$D$/$k;~4|$,$"$j!"@w?'BN?t$,B?$$$H$$$&$3$H$O!"$D$^$j(B
>telomere$B$N?t$bB?$$$H$$$&$o$1$G$9!#$7$?$,$C$F!"<B83$N:]$KET9g$,$$$$$H$$$&$N$,!"(B
>$BM}M3$@$C$?$h$&$K;W$$$^$9!#(B

$B%F%H%i%R%a%J$C$F%"%/%A%s$KFCD'$,$"$C$?$j$7$^$9!#(B

>telomere$B$N9=B$$O2<$N?^$N$h$&$K!"#3!GKvC<$,(Bover-hang$B$7$F$$$^$9!#(B
>
>     3'AACCCCAACCCC
>     5'TTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGG
>
>$B$9$k$HLdBj$O!"$I$&$d$C$F(Btemplate$B$J$7$G(BTTGGGG$B$N7+$jJV$7$r9g@.$9$k$N$+!"(B
>$B$H$$$&$3$H$K$J$j$^$9!#!JEvA3!"IaDL$N(BDNA polymerase$B$G$OIT2DG=!K(B
>
>$B$3$NLr3d$rC4$&!"Hs>o$KLLGr$$9ZAG$,$H$i$l$?$N$,!"3N$+#3G/A0$0$i$$$G!"(B
>$B$I$&LLGr$$$+$H$$$&$H!"$3$N(Btelomerase (telomere terminal transferase)$B$H$$$&(B
>$B$d$D$O!"(B159nt$B$N(BRNA$B$r$b$DCAGr(B-RNA$BJ#9gBN$G!"$=$N(BRNA$B$N$J$+$K(B 5'CAACCCCAA 3'
>$B$H$$$&G[Ns$,4^$^$l$F$$$k$H$$$&$N$G$9!#$9$k$H!"9M$($i$l$k%b%G%k$O!"(B
>
>$B#1!K(Btelomere$B$N(BTTGGGG$B$H!"(Btelomerase$B$N(BRNA$B$,1v4pBP$r7A@.$7(B
>$B#2!K(BRNA$B$r(Btemplate$B$K$7$F!"(BTTGGGG$B$r9g@.$9$k!#(B
>$B#3!K(Btemplate$B$N(BRNA$B$,%9%i%$%I$9$k!#(B
>$B#4!K#1!K$+$i#3!K$r7+$jJV$9(B
>
>$B#1!K(B         r3'AACCCCAAC 5'    $B#2!K(B          r3'AACCCCAAC 5'    
>   d5'----TTGGGGTTG                 d5'----TTGGGGTTGgggttg
>
>$B#3!K(B               r3'AACCCCAAC 5'     
>   d5'----TTGGGGTTGgggttg   
>                                                   
>$B$7$?$,$C$F!"(Btelomerase$B$O!c<+A0$G(BRNA-template$B$r$b$C$?5UE><L9ZAG$N0l<o!d(B
>$B$H$$$&$3$H$K$J$k$H;W$$$^$9!#B>$K$O$3$&$$$C$?Nc$O!"$[$H$s$ICN$i$l$F$$$J$$(B
>$B$N$G$O$J$$$G$7$g$&$+!#$^$"!"$=$l$@$1(Btelomere$B$,FCJL$J0UL#$r$b$D$H$$$&$3$H(B
>$B$J$N$G$7$g$&!#(B

$B$3$N<o$N9ZAG$N(BRNA-template$B$rG$0U$NG[Ns$KF~$l49$($k$3$H$O$G$-$k$N$G$7$g$&(B
$B$+!#Nc$($P(Br3'AAATTT5'$B$N$h$&$J$b$N$,(Btemplate$B$K$J$C$FH?I|G[Ns$r:n$i$;$?$i!"(B
$B0lK\$N@w?'BN$,4D>u$K%;%k%U%i%$%2!<%7%g%s$7$?$j$7$F!#(B

>$B$5$i$K!"(BTTGGGGTTGGGG$B$O!"!c(BG$B#4$D$GFC<l$J1v4pBP$r7A@.$7$F$$$k!d$H$+!"$3$N(B
>$BG[Ns$KFC0[E*$K7k9g$9$kCAGr$,$H$i$l$?!"$H$+$$$&OC$7$b$"$k$N$G$9$,!"$=$l$O(B
>$B$^$?<!$N5!2q$K$G$b!"!"!"!"$J$s$K$;$h!"@5>o$J$b$N$G$O!"9g@.$7$?$jJ,2r$7$?$j$H(B
>$B$$$C$?J?9U>uBV$H$$$&$o$1$G$O$J$/!"$`$7$mFC<l$J9=B$$GJ]8n$5$l$F$$$k$h$&$@!"(B
>$B$H$$$&0u>]$r$b$C$?5-21$,$"$j$^$9!#!J$$$m$$$m$J(Bmutant$B$G$O!"J?9U>uBV$H$$$&(B
>$B$b$N$b$"$C$?$h$&$K$*$b$$$^$9!#!K(B

$B%F%H%i%R%a%J$N$h$&$J@8J*$G(Btelomerase$B0dEA;R$r%?!<%2%F%#%s%0$7$FIT3h2=$7$?$i!"(B
$B$d$O$j@$Be$4$H$K@w?'BN$,C;$/$J$C$F$$$/$N$G$7$g$&$+!)!!9ZAG$N0dEA;R$O$H$i$l(B
$B$F$$$k$N$+$J!)!!<!$N5!2q$N$*OC$r$*BT$A$7$^$9!#(B

>$B$3$3?tG/$O(Bfollow$B$7$F$$$J$$$N$G!"$=$N8e$I$&$J$C$F$$$k$N$G$7$g$&$+!#(B

$B@h$[$I(BCurrent Contents with Abstracts$B$G:G6a#1%v7n$[$I$NO@J8$r%5!<%A$7$F$_$?(B
$B$i!"%R%H$NO@J8$@$1$G(B4$BJs=P$F$-$^$7$?!#$"$kIB5$$G%F%m%a%"$,C;$/$J$C$F$$$k$@$N(B
$BGr7lIB$N%j%s%Q5e$G%F%m%a%"$,C;$/$J$k$H$+$$$&$N$G$9!#3K7?$b:Y$+$/=R$Y$i$l$F(B
$B$$$F!"%2%N%`%W%m%8%'%/%H$,I,MW$K$J$k$o$1$@$H;W$$$^$7$?!#(B


$B!!!!!!!!!!!!!!!!!!CS!!0j@8!wM}2=3X8&5f=j!!F0J*!&:YK&%7%9%F%`8&5f<<(B
                                           fike@rkna50.riken.go.jp
                 TEL:048-462-1111(ext5042), FAX:048-462-4681(direct)