🚀 go-pugleaf

In article <2555@shzkrd.u-shizuoka-ken.ac.jp> hoshino@ss7.u-shizuoka-ken.ac.jp (Minoru HOSHINO) writes:
 |$B<B$O!"#D#N#A$NC<$O$I$&$J$C$F$$$k$N(B$B$G$7$g$&$+!)(B
 |$B$3$3$G$$$&#D#N#A$O??3K@8J*$N3K$KB8:_$9$k#D#N#A$r;X$7$F$$$^$9!#(B
 |$B;n834I$G07$C$F$$$k#c#D(B$B#N#A$N$h$&$J$b$N$G$O$"$j$^$;$s!#(B
 |$B$^$?!"Cj=P$7$F%:%?%:%?$K$J$C$F$$$k#D#N#A$G$b$"$j$^$;$s!#(B

$B#D#N#A$N(B$BKvC<!"FC$K??3K@8J*$N@w?'BNKvC<It!J%F%m%a%"!K$N(B
$B>\$7$$;EAH$_$K$D$$$F$O!"652J=q!J%o%H%=%s!X0dEA;R$NJ,;R(B$B@8J*3X!Y!K(B
$B$K$h$l$P!"$"$C$5$j$^$:!V$o$+$i$J$$!W$H=q$$$F$"$j$^$9!#(B
$B$,!"9=B$$O$I$&$b!"(B

	3'-AACCCC-5'
	5'-TTGGGG-3'

$B$H$$$&G[Ns$,BgNL$K7+$jJV$5$l$F$$$F!"$7$+$b$3$NC10L$rJd$&9ZAG$,(B
$BB8:_$7$F$$$F!"J#@=2aDx$G(B$B@w?'BN$,C;$/$J$C$F$7$^$&$N$rKI$$$G(B
$B$$$k$H$$$&2>@b$,8=:_M-NO$@$=$&$G$9!#(B

$B$\$m$\$m$3$\$lMn$A$F$$$/(BDNA$B!"(B$B$=$l$r0l=j7|L?$/$C$D$1$k9ZAG!"(B
$BN><T$N%P%i%s%9$GD9$5$rJ]$C$F$$$k$H$$$&%$%a!<%8$J$s$G$7$g$&$+!#(B

 |$B5?Ld(B$B#1!K$d$O$j!"Fs=E:?$O(B
 |$B#5!G!!#N#p#N#p!&!&!&!&!&!&#p#N#p!!#3!G(B
 |$B#3!G#p#N#p#N#p!&!&!&!&!&!&#p#N!!!!#5!G(B
 |$B$N$h$&$K!"#3!GKvC<$K$O%j%s;@4p$,IU$-!"#5!GKvC<$O%U%j!<(B
 |$B$J$N$+!)(B

$B$?$V$s!"J?9U>uBV$H$$$&$s$G$7$g$&(B$B$+!"$3$N7A$r4^$a$F(B
$B$$$m$$$m$JKvC<>uBV$,F15o$7$F$$$k%$%a!<%8$J$N$G$O!#(B

 |$B5?Ld#2!K>e$N$h$&$JFs=E:?$@$H(B$B!"C<$+$i$[$0$l$=$&$@$7!"(B
 |$B9ZAG$K>C2=$5$l$=$&$@$7!"$$$D$N$^$K$+#D#N#A$NC<$,(B
 |$BC;$/$J$C$F$$$-$=$&$J5$$,(B$B$7$^$9!#(B

$B$3$l$K4X$7$F$O!"2>@b;2>H!#(B

 |$B@8J*$O!"#D#N#A$NC<$KFCJL$J;E3]$r$7$F$$$k$N$G$7$g$&$+!)(B

$B??3K@8(B$BJ*$H8B$C$F$*$i$l$k$N$G!";d$J$s$>$h$j<ALd<T$NJ}$,(B
$B$3$N$X$s$O>\$7$$$N$G$O$H4*7+$C$?$j$7$F$^$9$,(B(^^;) 
$B@~(B$B>u$N@w?'BN$r$b$D%&%#%k%9$N0l<o$G$O!"KvC<$K%k!<%W9=B$$r(B
$B:NMQ$7$F$$$k$b$N$b$"$k$=$&$G!"$J$+$J$+9M$($F$$(B$B$k$_$?$$$G$9$M!#(B
$B!JC/$,!)(B(^^;)$B!K(B

======  $BEDCf=(=S!w#I#C#O#T(B
$B!!!!!!!!(Bhtanaka@icot.or.jp
$B!!!!!!!!(BGBA01223 (NIFTY)   ======

In article <HTANAKA.92Sep29161443@kappa.icot.or.jp> htanaka@icot.or.jp (Tanaka Hidetosi) writes:
 | $B#D#N#A$NKvC<!"FC$K??3K@8J*$N@w?'BNKvC<It!J%F%m%a%"!K$N(J
 | $B>\$7$$;EAH$_$K$D$$$F$O!"652J=q!J%o%H%=%s!X0dEA;R$NJ,;R@8J*3X!Y!K(J
 | $B$K$h$l$P!"$"$C$5$j$^$:!V$o$+$i$J$$!W$H=q$$$F$"$j$^$9!#(J

$B8=67$G$O!"$o$+$i$J$$$H$$$&$3$H$G$9$+!)!*<j85$N@82=3X$N652J=q$r(J
$B8+$F$b!"%/%m%^%A%s$N9=B$$J$I$O>\$7$/=q$$$F$"$k$N$K!"C<$C$3$N(J
$B$3$H$O$I$3$rA\$7$F$b8+$"$?$j$^$;$s!#$"$$$K$/%o%H%=%s$O(J
$BFI$s$G$$$^$;$s!#(J

 | $B$,!"9=B$$O$I$&$b!"(J
 | 
 | 	3'-AACCCC-5'
 | 	5'-TTGGGG-3'
 | 
 | $B$H$$$&G[Ns$,BgNL$K7+$jJV$5$l$F$$$F!"$7$+$b$3$NC10L$rJd$&9ZAG$,(J
 | $BB8:_$7$F$$$F!"J#@=2aDx$G@w?'BN$,C;$/$J$C$F$7$^$&$N$rKI$$$G(J
 | $B$$$k$H$$$&2>@b$,8=:_M-NO$@$=$&$G$9!#(J

$B2>@b$H$*$C$7$c$C$F$$$^$9$,!"$I$NJU$^$G<B83;v<B$H$7$F(J
$BL@$i$+$K$J$C$F$$$k$N$G$7$g$&$+!)$3$l$b!"%o%H%=%s$K(J
$B=q$$$F$"$j$^$9$+!)$=$l$H$bJL$NJ88%$G$7$g$&$+!)(J
$B=PE5$r$*65$(4j$($^$;$s$+!)D4$Y$F$_$?$$$H;W$$$^$9!#(J

$B>e$NG[Ns$N$h$&$@$H$9$k$H!"$+$J$jFC<l$J9ZAG!J72!)!K(J
$B$N$h$&$K8+$($^$9$M!#A[A|$r$?$/$^$7$/$9$k$H(J^_^;
$B$3$N9ZAG$O!"1v4p$NJB$SJ}$N7hDj8"$r$b$C$F$$$F!"(J
$B$^$:!"#D#N#A$NC<$G(J3'$BJ}8~$K$:$s$:$s#1K\:?$r$N$P$7$F$$$/!#(J
$B!J$3$l$O!"C<Kv9g@.9ZAG$NN`?d$G$9!K(J

$B#D#N#A#2K\:?(J
3'--------5'
5'--------TTGGGGCCCCAA--->3'

G$B$H(JC$B$N$H$3$m$G@^$jJV$;$P!"1v4pBP$,$G$-$F!":G8e$K(J
$B?-$P$7$?(J3'$BKvC<$H#D#N#A$N(J5'$BKvC<$r$/$C$D$1$F$7$^$&$H!"(J
$B!J%]%j%a%i!<%<3h@-$H%j%,!<%<3h@-$,I,MW!K(J

         $B"-(J $B$3$3$G$D$J$,$k!#(J
3'--------AACCCC|
5'--------TTGGGG| $B$3$3$G@^$l6J$,$k!#$M$8$l$J$$$H@^$l6J$,$l$J$$(J
$B!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!$+$bCN$l$J$$!#>.$5$J%k!<%W$K(J
$B!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!$J$C$F$$$k$+$bCN$l$J$$!#(J

$B$3$&$9$l$P!"%W%i%$%^!<$,$J$/$F$b#2K\:?$,$G$-$^$9!J$[$s$H!)!K(J
$B@^JV$7ItJ,$,1v4pBP$r:n$C$F%W%i%$%^!<E*$K$O$?$i$/!#(J
$B$G$9$+$i!"#D#N#A$K$OC<$,$J$/!"$J$,!A$$%k!<%W$K$J$C$F$$$k!*(J
$BEDCf$5$s$,<($5$l$?G[Ns$+$iF3$+$l$kO@M}!J!)!KE*$J?dO@$G$9!#(J
$BLdBj$O!"$I$&$d$C$F1v4pG[Ns$,>e$N$h$&$K$J$C$F$$$k$+$G$9$M!#(J

 | 
 | $B$\$m$\$m$3$\$lMn$A$F$$$/(JDNA$B!"$=$l$r0l=j7|L?$/$C$D$1$k9ZAG!"(J
 | $BN><T$N%P%i%s%9$GD9$5$rJ]$C$F$$$k$H$$$&%$%a!<%8$J$s$G$7$g$&$+!#(J
 | 
 |  |$B5?Ld#1!K$d$O$j!"Fs=E:?$O(J
 |  |$B#5!G!!#N#p#N#p!&!&!&!&!&!&#p#N#p!!#3!G(J
 |  |$B#3!G#p#N#p#N#p!&!&!&!&!&!&#p#N!!!!#5!G(J
 |  |$B$N$h$&$K!"#3!GKvC<$K$O%j%s;@4p$,IU$-!"#5!GKvC<$O%U%j!<(J
 |  |$B$J$N$+!)(J
 | 
 | $B$?$V$s!"J?9U>uBV$H$$$&$s$G$7$g$&$+!"$3$N7A$r4^$a$F(J
 | $B$$$m$$$m$JKvC<>uBV$,F15o$7$F$$$k%$%a!<%8$J$N$G$O!#(J

$B>e$N?dO@$,@5$7$1$l$P!"$3$N5?Ld$OL50UL#$K$J$j$^$9$M!#(J

 | 
 |  |$B5?Ld#2!K>e$N$h$&$JFs=E:?$@$H!"C<$+$i$[$0$l$=$&$@$7!"(J
 |  |$B9ZAG$K>C2=$5$l$=$&$@$7!"$$$D$N$^$K$+#D#N#A$NC<$,(J
 |  |$BC;$/$J$C$F$$$-$=$&$J5$$,$7$^$9!#(J
 | 
 | $B$3$l$K4X$7$F$O!"2>@b;2>H!#(J
 | 
 |  |$B@8J*$O!"#D#N#A$NC<$KFCJL$J;E3]$r$7$F$$$k$N$G$7$g$&$+!)(J
 | 
 | $B??3K@8J*$H8B$C$F$*$i$l$k$N$G!";d$J$s$>$h$j<ALd<T$NJ}$,(J
 | $B$3$N$X$s$O>\$7$$$N$G$O$H4*7+$C$?$j$7$F$^$9$,(J(^^;) 

$B$$$($$$(!"$H$s$G$b$"$j$^$;$s!J%^%8!K!#??3K$H863K$G$O(J
$B3K$N9=B$$,Bg$-$/0c$&$o$1$G$9$+$i!"F1$85!9=$b$"$l$P(J
$B0c$&5!9=$b$"$k$G$"$m$&$H$$$&$3$H$G!"C1=c$K8BDj$7$?$@$1$G$9!#(J

 | $B@~>u$N@w?'BN$r$b$D%&%#%k%9$N0l<o$G$O!"KvC<$K%k!<%W9=B$$r(J
 | $B:NMQ$7$F$$$k$b$N$b$"$k$=$&$G!"$J$+$J$+9M$($F$$$k$_$?$$$G$9$M!#(J
 | $B!JC/$,!)(J(^^;)$B!K(J

$B>e$N?dO@$@$H!"??3K@8J*$b%k!<%W$K$J$C$F$$$k!)!*(J

$B$3$N?dO@$,;v<B$H$"$C$F$$$kJ]>Z$O$I$3$K$b$"$j$^$;$s$,!"(J
$BEDCf$5$s$N<($5$l$?1v4pG[Ns$+$i!"L4$,KD$i$_$^$7$?!#(J

$B$G$b!"$3$l$,$[$s$H$&$@$H$b$C$H3Z$7$$$G$9$M!#(J

$B!t%R%HBh#2#1@w?'BND9OSIt$N0dEA;RCO?^$,40@.$7$?$=$&$G$9!#(J
$B!t$3$l$K!"#D#N#AKvC<LdBj$r2r$/80$O$J$$$G$7$g$&$+$M!#(J

---------
                             $B@1Ln!!L-!!(J(hoshino@u-shizuoka-ken.ac.jp)
 $B!!!!!!!!(J                              $B@E2,8)N)Bg3X!&@83h7r9/2J3X8&5f2J(J

In article <2564@shzkrd.u-shizuoka-ken.ac.jp> hoshino@ss7.u-shizuoka-ken.ac.jp (Minoru HOSHINO) writes:
 | | $B$,!"9=B$$O$I$&$b!"(B
 | | 
 | | 	3'-AACCCC-5'
 | | 	5'-TTGGGG-3'
 | | 
 | | $B$H$$$&G[Ns$,BgNL$K7+$jJV$5$l$F$$$F!"$7$+$b$3$NC10L$rJd$&9ZAG$,(B
 | | $BB8:_$7$F$$$F!"J#(B$B@=2aDx$G@w?'BN$,C;$/$J$C$F$7$^$&$N$rKI$$$G(B
 | | $B$$$k$H$$$&2>@b$,8=:_M-NO$@$=$&$G$9!#(B

 |$B2>@b$H$*$C$7$c(B$B$C$F$$$^$9$,!"$I$NJU$^$G<B83;v<B$H$7$F(B
 |$BL@$i$+$K$J$C$F$$$k$N$G$7$g$&$+!)$3$l$b!"%o%H%=%s$K(B
 |$B=q$$$F$"(B$B$j$^$9$+!)$=$l$H$bJL$NJ88%$G$7$g$&$+!)(B
 |$B=PE5$r$*65$(4j$($^$;$s$+!)D4$Y$F$_$?$$$H;W$$$^$9!#(B

$B%o%H%=%s(B$B$@$HBh#9>O$HBh#1#0>O!"FC$KBh#1#0>O$N:G8e$NJ}$G$9!#(B
$B$3$&$$$&9ZAG$O%F%H%i%R%a%J$G8+$D$+$C$?!"$H$"$j$^$9(B$B!#(B
$B;29MJ88%$O$?$V$s0J2<$N#2$D$G$9!#$^$:%o%H%=%s$r<h$j4:$($:FI$s$G(B
$B$b$i$C$?J}$,$$$$$G$7$g$&!#$J$s$;;d$O(B$B9b9;$N$H$-@8J*$O9%$-$8$c$J$/$F(B
$B#I#I$O<h$i$J$+$C$?E[$G$9$+$i!#!J$"!"G/$,$P$l$k$N$+$J$3$l!K(B

Shampay,J., Szostak,J.W. and Blackburn,E.H.:
"DNA Sequences of Teromeres Maintained in Yeast",
Nature 310:154-157 (1984).

Yao,M.-C., Zhu,S.-G. and Yao,C.-H.:
"The Amplification in Tetrahymena thermophilia: Formation of
Extrachromosomal Palindromic Genes Coding for rRNA",
Mol.Cell.Biol. 5:1260-1267 (1985).

 |$B!t(B$B%R%HBh#2#1@w?'BND9OSIt$N0dEA;RCO?^$,40@.$7$?$=$&$G$9!#(B
 |$B!t$3$l$K!"#D#N#AKvC<LdBj$r2r$/80$O$J$$$G$7$g(B$B$&$+$M!#(B

$B9ZJlBh#3@w?'BN$N(B 315357 bp (ds-DNA) $BG[Ns40A4FI$_$NJ}$b$J$+$J$+!#(B(^^)
16-MAR-1992 $B$G(B GenBank $B$K$b:\$C$F$^$9!#(B(YSCCHRIII, X59720)
$B!JCN$k?M$>CN$k!"Cx<T(B 150 $B?M$N(B 9 $B%Z!<%8O@J8!*!K(B

        1 cccacacacc acacccacac cacacccaca caccacacac accacaccca cacacccaca
       61 ccacaccaca cccacaccac acccacacac ccacacccac acaccacacc cacacacacc
      121 acacccacac acacccacac ccacacacca cacccacaca cacaccacac ccacacacac
      181 cacaccacac ccacaccaca cccacaccca cacaccacac ccacacccac accccacacc
      241 cacacaccac acccacacac accacaccca cacacaccca caccacaccc acacaccaca
      301 cccacacacc cacacccaca cacaccacac ccacaccaca cccacaccca cacacccaca
      361 ccctaacact accctaacac taccctattc taaccctgat tttacctgtc tcccaactta
      421 ctctccatta ccctacctct ccactcgtta ccctgtctga ttcaaccgta ccactcccaa
      481 ccaccatcca tctctctact tactaccacc aacccaccgt ccaccataac cgttaccctc

$B!JN,$5$J$$$HLBOG(B(^^;)$B!K(B

   315061 agcaggctga atggaaacta cgatataacg cagtagaggt cccccaagag ctggaaagaa
   315121 aatattggtt tgatagccag cactattcta tccagtgggc tcttgtacca ttttctgtgg
   315181 tatggctttg attcaactgc atacgtagtt aacgcgatca gccgcacatg ggcattatca
   315241 ttttcagggg tactggtaaa ccctattgtt tagaaagaat acctggtaag cgggtctgtc
   315301 gagtctttcc caactaattt tgaagaaaaa atctttacct agtttgagaa taatcgt

$B$5$!!"80$O$"$k$+!)!)(B(^^;)(^^;)

======  $BEDCf=(=S!w#I#C#O#T(B
$B!!!!!!!!(Bhtanaka@icot.or.jp
$B!!!!!!!!(BGBA01223 (NIFTY)   ======

$BCS!wM}2=3X8&5f=j$G$9!#(B

In article <HTANAKA.92Sep29161443@kappa.icot.or.jp> htanaka@icot.or.jp (Tanaka Hidetosi) writes:
>
>$B#D#N#A$NKvC<!"FC$K??3K@8J*$N@w?'BNKvC<It!J%F%m%a%"!K$N(B
>$B>\$7$$;EAH$_$K$D$$$F$O!"652J=q!J%o%H%=%s!X0dEA;R$NJ,;R@8J*3X!Y!K(B
>$B$K$h$l$P!"$"$C$5$j$^$:!V$o$+$i$J$$!W$H=q$$$F$"$j$^$9!#(B

$B!X0dEA;R$NJ,;R@8J*3X!JBh#4HG!K!Y$H!X:YK&$NJ,;R@8J*3X!JBh#2HG!K!Y$rFI$s$G(B
$B$_$^$7$?!#(B

>$B$,!"9=B$$O$I$&$b!"(B
>
>	3'-AACCCC-5'
>	5'-TTGGGG-3'
>
>$B$H$$$&G[Ns$,BgNL$K7+$jJV$5$l$F$$$F!"$7$+$b$3$NC10L$rJd$&9ZAG$,(B
>$BB8:_$7$F$$$F!"J#@=2aDx$G@w?'BN$,C;$/$J$C$F$7$^$&$N$rKI$$$G(B
>$B$$$k$H$$$&2>@b$,8=:_M-NO$@$=$&$G$9!#(B

$B!X:YK&$NJ,;R@8J*3X!Y$K$h$k$H#D#N#A%]%j%a%i!<%<$O#D#N#AJ#@=$N:]$K@~>u$N#D#N#A(B
$BJ,;R$NKvC<$r40A4$KJ#@=$9$k$3$H$,$G$-$J$$$N$G!"??3K@8J*$O%F%m%a%"(B($BCm(B)$B$J$kFC<l(B
$B$J1v4pG[Ns$rMQ0U$9$k$3$H$K$h$C$F40A4$JJ#@=$r2DG=$K$7$?$H=q$$$F$"$j$^$9!JF|K\(B
$B8lHG(Bp.484$B!"(Bp.519)$B!#(B

$BCm!K(B $B%F%m%a%"(B(telomere)$B!'@82=3X<-E5!JBh#2HG!K!"@8J*3X<-E5!JBh#2HG!K$*$h$S(B
$B@82=3XMQ8l<-E5$K$h$k$HKvC<>.N3$HLu$7$F$$$k$h$&$G$9!#(B

$B%F%m%a%"$NNc$H$7$F$O!"%F%H%i%R%a%J$NB>$K%7%m%$%L%J%:%J$N(B[TTTAGGG]$BH?I|!"%H%j(B
$B%Q%N%=!<%^$d%R%H$N(B[TTAGGG]$BH?I|!"9ZJl$N(B[TG(1-3)]$BH?I|$H$$$C$?$b$N$,<($5$l$F$$(B
$B$^$9!#$$$:$l$K$7$F$b#G$,B?$$$H$$$&FCD'$,$"$C$F%F%H%i%R%a%J$N%F%m%a%J!<%<$O(B
$B%F%m%a%"$N$3$NFCD'$KBP1~$7$F7k9g$9$k$H$$$&$3$H$G$9!#(B $B4p<A$O%L%/%l%*%7%I;0%j(B
$B%s;@!#%F%s%W%l!<%H$O;H$$$^$;$s!#(B

$B!X0dEA;R$NJ,;R@8J*3X!Y$G$O!"%F%m%a%J!<%<$O%F%m%a%"$N4pK\G[Ns$r%W%i%$%^!<$K$7(B
$B$F#3!GKvC<$K4pK\G[Ns$rIU$12C$($F$$$/$h$&$G$9$M!#$3$&$d$C$F?-D9$7$?#3!GKvC<$N(B
$B#D#N#A$r%F%s%W%l!<%H$K$7$F#D#N#A%]%j%a%i!<%<$,#5!GKvC<$r?-D9$5$;$F$$$/$,N>C<(B
$B$,B7$&$3$H$O$J$$$h$&$G$9!#$?$@$7#3!GKvC<$,@^$jJV$7$F@w?'BN$NKvC<$rJ]8n$9$k2D(B
$BG=@-$OH]Dj$G$-$^$;$s$,!#(B

>$B$\$m$\$m$3$\$lMn$A$F$$$/(BDNA$B!"$=$l$r0l=j7|L?$/$C$D$1$k9ZAG!"(B
>$BN><T$N%P%i%s%9$GD9$5$rJ]$C$F$$$k$H$$$&%$%a!<%8$J$s$G$7$g$&$+!#(B

$B$-$C$H$=$&$J$s$G$7$g$&$M!#$&$^$/$G$-$F$$$k$b$N$G$9$M!#(B

$B!!!!!!!!!!!!!!!!!!CS!!0j@8!wM}2=3X8&5f=j!!F0J*!&:YK&%7%9%F%`8&5f<<(B
                                           fike@rkna50.riken.go.jp
                 TEL:048-462-1111(ext5042), FAX:048-462-4681(direct)