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Thread View: sci.bio.immunocytochem
4 messages
4 total messages Started by Matt Ireland Mon, 30 Apr 2001 06:03
Immunohistochemistry on Pottasium Channels
#11
Author: Matt Ireland
Date: Mon, 30 Apr 2001 06:03
13 lines
1780 bytes
Dear Colleagues

I'm currently doing research on the potassium channel, the m-current.
The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this
stage, I would like to examine the distribution using fluorescent
antibodies for these gene subunits. Unfortunately, I have searched
almost every antibody company I can find, with no results. I recognise
that these newsgroups are spread across a number of different
disciplines in immunology, but if anyone is familiar with these
antibodies or where to acquire them, I�d be most grateful if you could
let me know.

With Thanks Matthew
Re: Immunohistochemistry on Pottasium Channels
#12
Author: Mark Haynes
Date: Tue, 01 May 2001 18:05
23 lines
2293 bytes
There are so many companies that sell abs.  If you know of any one who
subscribes to linscott's (it a antibody source database kinda thing that
can be searched) you should check there.  Otherwise, are the proteins
themselves available?  Is so maybe you could make some anti-sera with
rabbits or something like that.
good luck
markH

Matt Ireland wrote:

> Dear Colleagues
>
> I'm currently doing research on the potassium channel, the m-current.
> The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this
> stage, I would like to examine the distribution using fluorescent
> antibodies for these gene subunits. Unfortunately, I have searched
> almost every antibody company I can find, with no results. I recognise
> that these newsgroups are spread across a number of different
> disciplines in immunology, but if anyone is familiar with these
> antibodies or where to acquire them, I�d be most grateful if you could
> let me know.
>
> With Thanks Matthew
Re: Immunohistochemistry on Pottasium Channels
#13
Author: Henk Veldman
Date: Wed, 02 May 2001 06:37
31 lines
2255 bytes
Matt Ireland schreef:

> Dear Colleagues
>
> I'm currently doing research on the potassium channel, the m-current.
> The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this
> stage, I would like to examine the distribution using fluorescent
> antibodies for these gene subunits. Unfortunately, I have searched
> almost every antibody company I can find, with no results. I recognise
> that these newsgroups are spread across a number of different
> disciplines in immunology, but if anyone is familiar with these
> antibodies or where to acquire them, I�d be most grateful if you could
> let me know.
>
> With Thanks Matthew

I am not aware of any company that is selling antibodies against these
antigens. But things are developping fast, so there may some be around
somewhere. If so someone probably has used them in his research. The best
thing would be to do a Medline search (through Pubmed) on "pottasium
channel" and/or "KCNQ2" or KCNQ3". If something turns up the paper will
tell you were an antibody might be available. If not,  there probably isn't
any yet.
Good luck

Henk

--
H. Veldman
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)
Re: Immunohistochemistry on Pottasium Channels
#10
Author: Micki Kobylk
Date: Tue, 12 Jun 2001 17:48
37 lines
2889 bytes
Re channel antibodies - I have been recently looking into other channel
antibodies and have just received a "flyer" [catalog up-date] from Chemicon
"Antibodies for Channel Research" and there is an entire page of potassium
antibodies.  Perhaps contacting a representative from this company would
assist you in your search.  I have used several neurotransmitter/transporter
ab's from Chemicon with excellent result.  No, I don't work for this
company; but have been impressed with service, response to very specific
questions from their R & D dept and quality of products.  Good luck in your
search.
Micki Kobylk
University of Alaska Fairbanks
Mark Haynes <Mark.Haynes@Mail.TJU.Edu> wrote in message
news:3AEEFACE.DBAA7197@Mail.TJU.Edu...
> There are so many companies that sell abs.  If you know of any one who
> subscribes to linscott's (it a antibody source database kinda thing that
> can be searched) you should check there.  Otherwise, are the proteins
> themselves available?  Is so maybe you could make some anti-sera with
> rabbits or something like that.
> good luck
> markH
>
> Matt Ireland wrote:
>
> > Dear Colleagues
> >
> > I'm currently doing research on the potassium channel, the m-current.
> > The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this
> > stage, I would like to examine the distribution using fluorescent
> > antibodies for these gene subunits. Unfortunately, I have searched
> > almost every antibody company I can find, with no results. I recognise
> > that these newsgroups are spread across a number of different
> > disciplines in immunology, but if anyone is familiar with these
> > antibodies or where to acquire them, I'd be most grateful if you could
> > let me know.
> >
> > With Thanks Matthew
>
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