Thread View: sci.bio.immunocytochem
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Started by Matt Ireland
Mon, 30 Apr 2001 06:03
Immunohistochemistry on Pottasium Channels
Author: Matt Ireland
Date: Mon, 30 Apr 2001 06:03
Date: Mon, 30 Apr 2001 06:03
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Dear Colleagues I'm currently doing research on the potassium channel, the m-current. The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this stage, I would like to examine the distribution using fluorescent antibodies for these gene subunits. Unfortunately, I have searched almost every antibody company I can find, with no results. I recognise that these newsgroups are spread across a number of different disciplines in immunology, but if anyone is familiar with these antibodies or where to acquire them, I�d be most grateful if you could let me know. With Thanks Matthew
Re: Immunohistochemistry on Pottasium Channels
Author: Mark Haynes
Date: Tue, 01 May 2001 18:05
Date: Tue, 01 May 2001 18:05
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There are so many companies that sell abs. If you know of any one who subscribes to linscott's (it a antibody source database kinda thing that can be searched) you should check there. Otherwise, are the proteins themselves available? Is so maybe you could make some anti-sera with rabbits or something like that. good luck markH Matt Ireland wrote: > Dear Colleagues > > I'm currently doing research on the potassium channel, the m-current. > The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this > stage, I would like to examine the distribution using fluorescent > antibodies for these gene subunits. Unfortunately, I have searched > almost every antibody company I can find, with no results. I recognise > that these newsgroups are spread across a number of different > disciplines in immunology, but if anyone is familiar with these > antibodies or where to acquire them, I�d be most grateful if you could > let me know. > > With Thanks Matthew
Re: Immunohistochemistry on Pottasium Channels
Author: Henk Veldman
Date: Wed, 02 May 2001 06:37
Date: Wed, 02 May 2001 06:37
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Matt Ireland schreef: > Dear Colleagues > > I'm currently doing research on the potassium channel, the m-current. > The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this > stage, I would like to examine the distribution using fluorescent > antibodies for these gene subunits. Unfortunately, I have searched > almost every antibody company I can find, with no results. I recognise > that these newsgroups are spread across a number of different > disciplines in immunology, but if anyone is familiar with these > antibodies or where to acquire them, I�d be most grateful if you could > let me know. > > With Thanks Matthew I am not aware of any company that is selling antibodies against these antigens. But things are developping fast, so there may some be around somewhere. If so someone probably has used them in his research. The best thing would be to do a Medline search (through Pubmed) on "pottasium channel" and/or "KCNQ2" or KCNQ3". If something turns up the paper will tell you were an antibody might be available. If not, there probably isn't any yet. Good luck Henk -- H. Veldman Laboratory for Experimental Neurology (NMZ) University Medical Center Utrecht (AZU)
Re: Immunohistochemistry on Pottasium Channels
Author: Micki Kobylk
Date: Tue, 12 Jun 2001 17:48
Date: Tue, 12 Jun 2001 17:48
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Re channel antibodies - I have been recently looking into other channel antibodies and have just received a "flyer" [catalog up-date] from Chemicon "Antibodies for Channel Research" and there is an entire page of potassium antibodies. Perhaps contacting a representative from this company would assist you in your search. I have used several neurotransmitter/transporter ab's from Chemicon with excellent result. No, I don't work for this company; but have been impressed with service, response to very specific questions from their R & D dept and quality of products. Good luck in your search. Micki Kobylk University of Alaska Fairbanks Mark Haynes <Mark.Haynes@Mail.TJU.Edu> wrote in message news:3AEEFACE.DBAA7197@Mail.TJU.Edu... > There are so many companies that sell abs. If you know of any one who > subscribes to linscott's (it a antibody source database kinda thing that > can be searched) you should check there. Otherwise, are the proteins > themselves available? Is so maybe you could make some anti-sera with > rabbits or something like that. > good luck > markH > > Matt Ireland wrote: > > > Dear Colleagues > > > > I'm currently doing research on the potassium channel, the m-current. > > The current is comprised of two gene subunits, KCNQ2 and KCNQ3. At this > > stage, I would like to examine the distribution using fluorescent > > antibodies for these gene subunits. Unfortunately, I have searched > > almost every antibody company I can find, with no results. I recognise > > that these newsgroups are spread across a number of different > > disciplines in immunology, but if anyone is familiar with these > > antibodies or where to acquire them, I'd be most grateful if you could > > let me know. > > > > With Thanks Matthew >
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