Article View: sci.bio.microbiology
Article #232Re: weird bacteria - Update
From: Scott J. Coutts
Date: Fri, 07 Mar 2003 11:31
Date: Fri, 07 Mar 2003 11:31
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Trond Erik Vee Aune wrote: >> I'd recommend the 6% agar trick first of all. It will also make those >> colonies smaller, too. Spread them onto a plate. >> > > But how with this help me separate them? It'll only prevent the swarmers > from spreading. I'll still have the two strains together. > Yup, that's the idea. I assume you know how to streak a plate to obtain single colonies? I.e. streak an initial inoculum over a section of the plate, flame your loop, do a set of streakes coming from the first incolulation point, flame the loop, set of streaks from first set of streaks, flame etc etc. Otherwise, do a set of serial dilutions of your broth, and plate out 100uL of the dilutions on your plates. Some of them should end up with very few single colonies. -- Scott J. Coutts -------------------------------------------------------------------------- Bacterial Pathogenesis Research Group Department of Microbiology Ph [+61 3 9905 4838] PO Box 53 Fax [+61 3 9905 4811] Monash University, 3800, Austrlalia --------------------------------------------------------------------------
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 104764,e5a6cb838f6e55e1 X-Google-Attributes: gid104764,public X-Google-Thread: f6a65,e5a6cb838f6e55e1 X-Google-Attributes: gidf6a65,public X-Google-ArrivalTime: 2003-03-07 03:31:17 PST Path: arc1-ca.pugleaf.net!archiver1.google.com!news1.google.com!newsfeed.stanford.edu!logbridge.uoregon.edu!arclight.uoregon.edu!enews.sgi.com!news-out.spamkiller.net!propagator2-maxim!news-in.spamkiller.net!lon-transit.news.telstra.net!news.telstra.net!news-server.bigpond.net.au!not-for-mail Message-ID: <3E6883CC.9040508@med.monash.edu.au> From: "Scott J. Coutts" <scott.coutts@med.monash.edu.au> User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.0.2) Gecko/20021120 Netscape/7.01 X-Accept-Language: en-us, en MIME-Version: 1.0 Newsgroups: sci.bio.microbiology,bionet.microbiology Subject: Re: weird bacteria - Update References: <3E5E1832.7040302@biotech.REMOVETHISBEFOREREPLYING.ntnu.no> <vuywujllf4u.fsf@suspiria.ai.mit.edu> <3E5E9184.3070903@chembio.NOSPAM.ntnu.no> <3E5F11C5.9060107@med.monash.edu.au> <3E5F1556.7040703@biotech.REMOVETHISBEFOREREPLYING.ntnu.no> <3E605AF4.8010606@med.monash.edu.au> <3E625E1F.2050809@chembio.NOSPAM.ntnu.no> <b3tt06$b4i$1@knossos.btinternet.com> <3E62F0A3.4050407@med.monash.edu.au> <3E671C7D.2020408@biotech.REMOVETHISBEFOREREPLYING.ntnu.no> <3E686B95.4020205@med.monash.edu.au> <3E686EE1.9090806@biotech.REMOVETHISBEFOREREPLYING.ntnu.no> Content-Type: text/plain; charset=us-ascii; format=flowed Content-Transfer-Encoding: 7bit Lines: 30 Date: Fri, 07 Mar 2003 11:31:16 GMT NNTP-Posting-Host: 144.132.17.73 X-Complaints-To: news@bigpond.net.au X-Trace: news-server.bigpond.net.au 1047036676 144.132.17.73 (Fri, 07 Mar 2003 22:31:16 EST) NNTP-Posting-Date: Fri, 07 Mar 2003 22:31:16 EST Organization: BigPond Internet Services (http://www.bigpond.net.au)