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Article #263Re: Staining of flagella
From: Richard Oeffner
Date: Fri, 14 Sep 2001 19:31
Date: Fri, 14 Sep 2001 19:31
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I Know that I have seen a kit for flagellar staining, but not sure where to get it. It was not fluoresence based, but worked fairly well under a normal scope. Richard Oeffner Cal State San Marcos Wolfgang Wenzel wrote: > Hi Gerhard, > I remember a protocoll using Nano Orange from Molecular probes published > by H.P. Grossard and F. Azam. Never tried it myself. Hope it helps > > Wolfgang > > gerhard schrieb: > > > > Hi everyone > > > > I would like to detect the presence of flagella on bacteria, > > preferentially by fluorescence. > > > > Does anyone know of a flagellum specific fluorescent stain, perhaps an > > antibody that recognises flagella of a variety of species? > > > > Thanks in advance > > > > Gerhard Nebe-von-Caron
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: f6a65,42205560993d4b4e X-Google-Attributes: gidf6a65,public X-Google-Thread: 10a0d3,42205560993d4b4e X-Google-Attributes: gid10a0d3,public X-Google-Thread: feea0,42205560993d4b4e X-Google-Attributes: gidfeea0,public X-Google-Thread: 104764,42205560993d4b4e X-Google-Attributes: gid104764,public X-Google-ArrivalTime: 2001-09-14 14:13:06 PST Path: arc1-ca.pugleaf.net!archiver1.google.com!newsfeed.google.com!newsfeed.stanford.edu!newsfeed.berkeley.edu!ucberkeley!ihnp4.ucsd.edu!usc.edu!newshub.csu.net!news1.csusm.edu!not-for-mail From: Richard Oeffner <roeffner@csusm.edu> Newsgroups: sci.bio.microbiology,bionet.microbiology,sci.bio.immunocytochem,sci.bio.ecology Subject: Re: Staining of flagella Date: Fri, 14 Sep 2001 12:31:44 -0700 Organization: California State University San Marcos Lines: 29 Message-ID: <3BA25B20.AC1C411C@csusm.edu> References: <4005dc5d.0109071943.2d7d4c24@posting.google.com> <3BA1ED6D.C1131B38@linux.zrz.tu-berlin.de> NNTP-Posting-Host: sanmarcos.csusm.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.61 [en] (Win98; I) X-Accept-Language: en