Thread View: sci.bio.microbiology
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Started by Adam Lawrence
Thu, 02 Oct 2003 13:49
Flourescent stain for Ralstonia?
Author: Adam Lawrence
Date: Thu, 02 Oct 2003 13:49
Date: Thu, 02 Oct 2003 13:49
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Hi- Does anyone know of a blue or green fluorescent stain that will selectively stain the cell wall or cell membrane of gram-negative bacteria (Ralstonia eutropha)? Or does anyone have any guesses on stains that might work or companies I might try? I struck out with Molecular Probes and google is driving me nuts! Thanks! Adam
Re: Flourescent stain for Ralstonia?
Author: Captain Oates
Date: Thu, 02 Oct 2003 15:52
Date: Thu, 02 Oct 2003 15:52
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You could try Vector they're good for cell surface staining: http://www.vectorlabs.com/ Alex On Thu, 02 Oct 2003 09:49:42 -0400, Adam Lawrence <adamlaw@yahooFNORD.com> wrote: >Hi- > >Does anyone know of a blue or green fluorescent stain that will >selectively stain the cell wall or cell membrane of gram-negative >bacteria (Ralstonia eutropha)? Or does anyone have any guesses on >stains that might work or companies I might try? I struck out with >Molecular Probes and google is driving me nuts! > >Thanks! >Adam
Re: Flourescent stain for Ralstonia?
Author: EK
Date: Thu, 02 Oct 2003 16:57
Date: Thu, 02 Oct 2003 16:57
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Group of Alexander Steinbuchel has been working on developing methods of staining bacteria, producing polyhydroxyalkanoates and other inclusion bodies. I remember, Nile Blue, Nile Red, as well as tetrazolium violet worked quite well. Check out e.g. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9914303&dopt=Abstract Emir "Adam Lawrence" <adamlaw@yahooFNORD.com> wrote in message news:3f7c2cf6$0$578$b45e6eb0@senator-bedfellow.mit.edu... > Hi- > > Does anyone know of a blue or green fluorescent stain that will > selectively stain the cell wall or cell membrane of gram-negative > bacteria (Ralstonia eutropha)? Or does anyone have any guesses on > stains that might work or companies I might try? I struck out with > Molecular Probes and google is driving me nuts! > > Thanks! > Adam >
Re: Flourescent stain for Ralstonia?
Author: Scott Coutts
Date: Thu, 02 Oct 2003 23:40
Date: Thu, 02 Oct 2003 23:40
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What do you need to do with them? Does it have to be the membrane itself? or just the 'perimiter' of the cell? Does it suit your application to do an antibody mediated stain? Because if you cant find anything else, then you could use anti-LPS antibodies. You can get lipids that will incorporate into membranes but I'm not sure what labels you can get attached to them and unfortunately I dont have the reference for it! Adam Lawrence wrote: > Hi- > > Does anyone know of a blue or green fluorescent stain that will > selectively stain the cell wall or cell membrane of gram-negative > bacteria (Ralstonia eutropha)? Or does anyone have any guesses on > stains that might work or companies I might try? I struck out with > Molecular Probes and google is driving me nuts! > > Thanks! > Adam >
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