🚀 go-pugleaf

hi,
good to see the results at last, the sequencing does suggest that as 
predicted its not gram negative and does indeed belong to bacillus. i 
suggested that it is a bacillus type rather than a gram negative, and 
dear geneticist, it does confirm that experience can save u some amount 
of work when u have foresight to do so...
cheers with the results
subhash
PS: whats the thing with these bacteria being from ganges???

Trond Erik Vee Aune wrote:
> Hi all,
> 
> I just received the sequencing data of the "wierd bacterium" we've been 
> talking about earlier. Unfortunately, the sequence was not very good, 
> but I did get what I thnik is convincing results when blasting. I have 
> pasted the sequence so that you can check it out yourselves if you're 
> interested.
> 
> Anyway, I got very good match against Paenibacillus alvei. According to 
> web-resources this genus do produce different types of antibiotics as 
> well as being able to swarm in different ways 
> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange thing 
> is that it is not a gram negative bug as I thought after staining, but 
> one of you microbiologists suggested that I was a Bacillus strain, so I 
> guess we geneticists are much better at sequencing than staining ;) 
> Anyway, I'm pretty pleased that I finally got an identification, but of 
> course a little disappointed that it wasn't a new bacterium
> 
> Forward sequence:
> GGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTGATGGAGTGCTTGCACTCCTGATGGTTAGCGGC 
> 
> GGACGGGTGAGTAACACGTACGTAACCTGCCCATAAGACTGGGATAACCCACNGAAACGTGAGCTANTACCANATNGGCN 
> 
> TTTTCCTCGCATGAGGGAAATNAGAAAGGCGGATCAATCTGTCNCTTATGGATGGACCTGCGGNGCATTATCTAGTTGGT 
> 
> GAGGTAACGGCTNACCAAGGCGACGATGCGTNCCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGANACACGGTCCC 
> 
> AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTNTGACGGAGCAACGCCGTCGTGAGTGATNA 
> 
> ANGTTTTCNGATCGTAAAGCTCTGNTGCCANGGAANAACGCCTAGGAGAGTNACTNCTCTTAGGGNGACGGNNCCTGAGA 
> 
> AGAAAGCCCCTNCTAACTACNTGCCANCAGCCGNGGTANTACGTANGGGGCGAGCGTNNNCCANANTTATTNGGCTTAAA 
> 
> GCGCGCNCAGGCAGNAATNTAAGTTGGGTGTTTAAACCTAGGGCTCAACCTTGGGNCGCATNCAAAACTGCATANCTNGA 
> 
> NTACAGAAAGAGGAAAGNGGAATNCCNCGTGTTCCNGNGAAATGCGTANAGATNTCNAGGAACACNATTGN
> 
> 
> Reverse sequence:
> GGNACCCCAATCATCTACCCCACCTTCGGCGGCTGGCNCCTTGCGGTTACCTCACCGACTTCGGGTGTTGTAAACTCTCG 
> 
> TGGTGTGACGGGCGGNGTGTACAAGACCCGGGAACGTATTCACCGCTGGCATGCTGATCCGCGATTACTAGCAATTCCGA 
> 
> CTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGACTGGCTTTTTAGGATTGGCTNCACCTCGCGGNTTCGCTTC 
> 
> CCGATGTACCAGCCATTGTAGNACGTGNGTAGCCCAGGTCATAAGGGGCATGATGATTNGANNTNATNCTCACCTTCCTC 
> 
> CGGTACTGTCACCGGCAGGCACTNTAGAGTGNCCAACTGAATGCTGGCAANTAAAGANAAAGGTTGCGCTCGTGAGCGGG 
> 
> ACTTAACCCAACATTTNACAACANNAGCTGACGACAACCATNCACNACCNGANTANAAATGTCCNNANAGAAAGCCCTAT 
> 
> CTCTAGAACGNNCCTTCATATNTCAAGACCTGGTAAGGNTNTTNCCGTTGCTTCNAATTTNACCCCATACTTCACTGCNT 
> 
> TTNCCNGCNCCCCGCNCGTTCCTTTTAGNTTTCAAGTCTTTNCGCACCGTGCTTCCCCACGCGGAATGCTTTAATGANTT 
> 
> ACTTTCTNCCCCAAGGGAAATAGAAAACCCGNAATACCNAGTCTTNCTTGNTATACCNGCTNGGGCTTNCGGGGNATCNA 
> 
> ATNCCGTTTGCTCCNNCTTCCTTTCTCCNCNTNNCNNNTNGNTNCCNCCCNANAGGCNNCTTCCCCNATGGGATNCCTCN 
> 
> CNTNCTCCNCTAGNNCCGNAAACTNGGTAAGAAGATNNTTTTTCTNNNAACNAGTAANTCTAAACGNACCACNNGGGATN 
> 
> GGNAAAATAANTAATANANANAGGNACTAGACCAAGNTNNNGAGATNNTACCNAAAANNAANTTAGGGATNAGANANAGN 
> 
> NTTTTANACTACT
> 
> If you have any more information about this bacterium, please tell me, 
> I'm interested :)
> 
> Regards,
> Trond Erik
> 
> 

Subhash Kulkarni wrote:
> hi,
> good to see the results at last, the sequencing does suggest that as 
> predicted its not gram negative and does indeed belong to bacillus. i 
> suggested that it is a bacillus type rather than a gram negative, and 
> dear geneticist, it does confirm that experience can save u some amount 
> of work when u have foresight to do so...

:) Well, even if i did get a convincing stain I would have done the 
sequencing, I would never settle for just stain information.

> cheers with the results
> subhash
> PS: whats the thing with these bacteria being from ganges???

Just a friend of mine who brought some water sample fra Ganges, 
something I've been playing around with on my spare time. Well, I 
haven't been "playing around" with the water considering all the 
potential harmful bugs that are probably in there ;)...But I have done 
som tests to establish what kind of microorganisms is in the water.

Trond Erik

> 
> Trond Erik Vee Aune wrote:
> 
>> Hi all,
>>
>> I just received the sequencing data of the "wierd bacterium" we've 
>> been talking about earlier. Unfortunately, the sequence was not very 
>> good, but I did get what I thnik is convincing results when blasting. 
>> I have pasted the sequence so that you can check it out yourselves if 
>> you're interested.
>>
>> Anyway, I got very good match against Paenibacillus alvei. According 
>> to web-resources this genus do produce different types of antibiotics 
>> as well as being able to swarm in different ways 
>> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange 
>> thing is that it is not a gram negative bug as I thought after 
>> staining, but one of you microbiologists suggested that I was a 
>> Bacillus strain, so I guess we geneticists are much better at 
>> sequencing than staining ;) Anyway, I'm pretty pleased that I finally 
>> got an identification, but of course a little disappointed that it 
>> wasn't a new bacterium
>>
>> Forward sequence:
>> GGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTGATGGAGTGCTTGCACTCCTGATGGTTAGCGGC 
>>
>> GGACGGGTGAGTAACACGTACGTAACCTGCCCATAAGACTGGGATAACCCACNGAAACGTGAGCTANTACCANATNGGCN 
>>
>> TTTTCCTCGCATGAGGGAAATNAGAAAGGCGGATCAATCTGTCNCTTATGGATGGACCTGCGGNGCATTATCTAGTTGGT 
>>
>> GAGGTAACGGCTNACCAAGGCGACGATGCGTNCCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGANACACGGTCCC 
>>
>> AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTNTGACGGAGCAACGCCGTCGTGAGTGATNA 
>>
>> ANGTTTTCNGATCGTAAAGCTCTGNTGCCANGGAANAACGCCTAGGAGAGTNACTNCTCTTAGGGNGACGGNNCCTGAGA 
>>
>> AGAAAGCCCCTNCTAACTACNTGCCANCAGCCGNGGTANTACGTANGGGGCGAGCGTNNNCCANANTTATTNGGCTTAAA 
>>
>> GCGCGCNCAGGCAGNAATNTAAGTTGGGTGTTTAAACCTAGGGCTCAACCTTGGGNCGCATNCAAAACTGCATANCTNGA 
>>
>> NTACAGAAAGAGGAAAGNGGAATNCCNCGTGTTCCNGNGAAATGCGTANAGATNTCNAGGAACACNATTGN
>>
>>
>> Reverse sequence:
>> GGNACCCCAATCATCTACCCCACCTTCGGCGGCTGGCNCCTTGCGGTTACCTCACCGACTTCGGGTGTTGTAAACTCTCG 
>>
>> TGGTGTGACGGGCGGNGTGTACAAGACCCGGGAACGTATTCACCGCTGGCATGCTGATCCGCGATTACTAGCAATTCCGA 
>>
>> CTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGACTGGCTTTTTAGGATTGGCTNCACCTCGCGGNTTCGCTTC 
>>
>> CCGATGTACCAGCCATTGTAGNACGTGNGTAGCCCAGGTCATAAGGGGCATGATGATTNGANNTNATNCTCACCTTCCTC 
>>
>> CGGTACTGTCACCGGCAGGCACTNTAGAGTGNCCAACTGAATGCTGGCAANTAAAGANAAAGGTTGCGCTCGTGAGCGGG 
>>
>> ACTTAACCCAACATTTNACAACANNAGCTGACGACAACCATNCACNACCNGANTANAAATGTCCNNANAGAAAGCCCTAT 
>>
>> CTCTAGAACGNNCCTTCATATNTCAAGACCTGGTAAGGNTNTTNCCGTTGCTTCNAATTTNACCCCATACTTCACTGCNT 
>>
>> TTNCCNGCNCCCCGCNCGTTCCTTTTAGNTTTCAAGTCTTTNCGCACCGTGCTTCCCCACGCGGAATGCTTTAATGANTT 
>>
>> ACTTTCTNCCCCAAGGGAAATAGAAAACCCGNAATACCNAGTCTTNCTTGNTATACCNGCTNGGGCTTNCGGGGNATCNA 
>>
>> ATNCCGTTTGCTCCNNCTTCCTTTCTCCNCNTNNCNNNTNGNTNCCNCCCNANAGGCNNCTTCCCCNATGGGATNCCTCN 
>>
>> CNTNCTCCNCTAGNNCCGNAAACTNGGTAAGAAGATNNTTTTTCTNNNAACNAGTAANTCTAAACGNACCACNNGGGATN 
>>
>> GGNAAAATAANTAATANANANAGGNACTAGACCAAGNTNNNGAGATNNTACCNAAAANNAANTTAGGGATNAGANANAGN 
>>
>> NTTTTANACTACT
>>
>> If you have any more information about this bacterium, please tell me, 
>> I'm interested :)
>>
>> Regards,
>> Trond Erik
>>
>>
> 


-- 
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun@biotech.ntnu.no
http://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen

Hi,

Congratulations on the ID. Remind us, did your bug have flagella ? P. alvei
is supposed to be flagellated... It is a common beehive parasite if memory
serves right, it can be found on dead bee larvae. It sporulates with spores
positioned in almost ordered rows. Sorry to hear that your bug isn't brand
new...

Artem


"Trond Erik Vee Aune" <trondaun@biotech.REMOVETHISBEFOREREPLYING.ntnu.no>
wrote in message
news:3EAD0F42.2000002@biotech.REMOVETHISBEFOREREPLYING.ntnu.no...
> Hi all,
>
> I just received the sequencing data of the "wierd bacterium" we've been
> talking about earlier. Unfortunately, the sequence was not very good,
> but I did get what I thnik is convincing results when blasting. I have
> pasted the sequence so that you can check it out yourselves if you're
> interested.
>
> Anyway, I got very good match against Paenibacillus alvei. According to
> web-resources this genus do produce different types of antibiotics as
> well as being able to swarm in different ways
> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange thing
> is that it is not a gram negative bug as I thought after staining, but
> one of you microbiologists suggested that I was a Bacillus strain, so I
> guess we geneticists are much better at sequencing than staining ;)
> Anyway, I'm pretty pleased that I finally got an identification, but of
> course a little disappointed that it wasn't a new bacterium
>
> Forward sequence:
>
GGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTGATGGAGTGCTTGCACTCCTGATGGTTAG
CGGC
>
GGACGGGTGAGTAACACGTACGTAACCTGCCCATAAGACTGGGATAACCCACNGAAACGTGAGCTANTACCANATN
GGCN
>
TTTTCCTCGCATGAGGGAAATNAGAAAGGCGGATCAATCTGTCNCTTATGGATGGACCTGCGGNGCATTATCTAGT
TGGT
>
GAGGTAACGGCTNACCAAGGCGACGATGCGTNCCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGANACACGG
TCCC
>
AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTNTGACGGAGCAACGCCGTCGTGAGTG
ATNA
>
ANGTTTTCNGATCGTAAAGCTCTGNTGCCANGGAANAACGCCTAGGAGAGTNACTNCTCTTAGGGNGACGGNNCCT
GAGA
>
AGAAAGCCCCTNCTAACTACNTGCCANCAGCCGNGGTANTACGTANGGGGCGAGCGTNNNCCANANTTATTNGGCT
TAAA
>
GCGCGCNCAGGCAGNAATNTAAGTTGGGTGTTTAAACCTAGGGCTCAACCTTGGGNCGCATNCAAAACTGCATANC
TNGA
> NTACAGAAAGAGGAAAGNGGAATNCCNCGTGTTCCNGNGAAATGCGTANAGATNTCNAGGAACACNATTGN
>
>
> Reverse sequence:
>
GGNACCCCAATCATCTACCCCACCTTCGGCGGCTGGCNCCTTGCGGTTACCTCACCGACTTCGGGTGTTGTAAACT
CTCG
>
TGGTGTGACGGGCGGNGTGTACAAGACCCGGGAACGTATTCACCGCTGGCATGCTGATCCGCGATTACTAGCAATT
CCGA
>
CTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGACTGGCTTTTTAGGATTGGCTNCACCTCGCGGNTTCG
CTTC
>
CCGATGTACCAGCCATTGTAGNACGTGNGTAGCCCAGGTCATAAGGGGCATGATGATTNGANNTNATNCTCACCTT
CCTC
>
CGGTACTGTCACCGGCAGGCACTNTAGAGTGNCCAACTGAATGCTGGCAANTAAAGANAAAGGTTGCGCTCGTGAG
CGGG
>
ACTTAACCCAACATTTNACAACANNAGCTGACGACAACCATNCACNACCNGANTANAAATGTCCNNANAGAAAGCC
CTAT
>
CTCTAGAACGNNCCTTCATATNTCAAGACCTGGTAAGGNTNTTNCCGTTGCTTCNAATTTNACCCCATACTTCACT
GCNT
>
TTNCCNGCNCCCCGCNCGTTCCTTTTAGNTTTCAAGTCTTTNCGCACCGTGCTTCCCCACGCGGAATGCTTTAATG
ANTT
>
ACTTTCTNCCCCAAGGGAAATAGAAAACCCGNAATACCNAGTCTTNCTTGNTATACCNGCTNGGGCTTNCGGGGNA
TCNA
>
ATNCCGTTTGCTCCNNCTTCCTTTCTCCNCNTNNCNNNTNGNTNCCNCCCNANAGGCNNCTTCCCCNATGGGATNC
CTCN
>
CNTNCTCCNCTAGNNCCGNAAACTNGGTAAGAAGATNNTTTTTCTNNNAACNAGTAANTCTAAACGNACCACNNGG
GATN
>
GGNAAAATAANTAATANANANAGGNACTAGACCAAGNTNNNGAGATNNTACCNAAAANNAANTTAGGGATNAGANA
NAGN
> NTTTTANACTACT
>
> If you have any more information about this bacterium, please tell me,
> I'm interested :)
>
> Regards,
> Trond Erik
>
>
> --
> Trond Erik Vee Aune
> Department of Biotechnology
> Norwegian University of Science and Technology
> Email: trondaun@biotech.ntnu.no
> http://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen
>

Artem Evdokimov wrote:
> Hi,
> 
> Congratulations on the ID. Remind us, did your bug have flagella ? P. alvei
> is supposed to be flagellated... It is a common beehive parasite if memory
> serves right, it can be found on dead bee larvae. It sporulates with spores
> positioned in almost ordered rows. Sorry to hear that your bug isn't brand
> new...

Yes, it was highly motile and swarmed in a very fascinating way. That's 
why I decided to identify it. I looked for spores in the microscope, but 
couldn't see any, so maybe the cells were in the wrong growth phase.

Trond Erik

> 
> Artem
> 
> 
> "Trond Erik Vee Aune" <trondaun@biotech.REMOVETHISBEFOREREPLYING.ntnu.no>
> wrote in message
> news:3EAD0F42.2000002@biotech.REMOVETHISBEFOREREPLYING.ntnu.no...
> 
>>Hi all,
>>
>>I just received the sequencing data of the "wierd bacterium" we've been
>>talking about earlier. Unfortunately, the sequence was not very good,
>>but I did get what I thnik is convincing results when blasting. I have
>>pasted the sequence so that you can check it out yourselves if you're
>>interested.
>>
>>Anyway, I got very good match against Paenibacillus alvei. According to
>>web-resources this genus do produce different types of antibiotics as
>>well as being able to swarm in different ways
>>(http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange thing
>>is that it is not a gram negative bug as I thought after staining, but
>>one of you microbiologists suggested that I was a Bacillus strain, so I
>>guess we geneticists are much better at sequencing than staining ;)
>>Anyway, I'm pretty pleased that I finally got an identification, but of
>>course a little disappointed that it wasn't a new bacterium
>>
>>Forward sequence:
>>
> 
> GGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTGATGGAGTGCTTGCACTCCTGATGGTTAG
> CGGC
> 
> GGACGGGTGAGTAACACGTACGTAACCTGCCCATAAGACTGGGATAACCCACNGAAACGTGAGCTANTACCANATN
> GGCN
> 
> TTTTCCTCGCATGAGGGAAATNAGAAAGGCGGATCAATCTGTCNCTTATGGATGGACCTGCGGNGCATTATCTAGT
> TGGT
> 
> GAGGTAACGGCTNACCAAGGCGACGATGCGTNCCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGANACACGG
> TCCC
> 
> AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTNTGACGGAGCAACGCCGTCGTGAGTG
> ATNA
> 
> ANGTTTTCNGATCGTAAAGCTCTGNTGCCANGGAANAACGCCTAGGAGAGTNACTNCTCTTAGGGNGACGGNNCCT
> GAGA
> 
> AGAAAGCCCCTNCTAACTACNTGCCANCAGCCGNGGTANTACGTANGGGGCGAGCGTNNNCCANANTTATTNGGCT
> TAAA
> 
> GCGCGCNCAGGCAGNAATNTAAGTTGGGTGTTTAAACCTAGGGCTCAACCTTGGGNCGCATNCAAAACTGCATANC
> TNGA
> 
>>NTACAGAAAGAGGAAAGNGGAATNCCNCGTGTTCCNGNGAAATGCGTANAGATNTCNAGGAACACNATTGN
>>
>>
>>Reverse sequence:
>>
> 
> GGNACCCCAATCATCTACCCCACCTTCGGCGGCTGGCNCCTTGCGGTTACCTCACCGACTTCGGGTGTTGTAAACT
> CTCG
> 
> TGGTGTGACGGGCGGNGTGTACAAGACCCGGGAACGTATTCACCGCTGGCATGCTGATCCGCGATTACTAGCAATT
> CCGA
> 
> CTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGACTGGCTTTTTAGGATTGGCTNCACCTCGCGGNTTCG
> CTTC
> 
> CCGATGTACCAGCCATTGTAGNACGTGNGTAGCCCAGGTCATAAGGGGCATGATGATTNGANNTNATNCTCACCTT
> CCTC
> 
> CGGTACTGTCACCGGCAGGCACTNTAGAGTGNCCAACTGAATGCTGGCAANTAAAGANAAAGGTTGCGCTCGTGAG
> CGGG
> 
> ACTTAACCCAACATTTNACAACANNAGCTGACGACAACCATNCACNACCNGANTANAAATGTCCNNANAGAAAGCC
> CTAT
> 
> CTCTAGAACGNNCCTTCATATNTCAAGACCTGGTAAGGNTNTTNCCGTTGCTTCNAATTTNACCCCATACTTCACT
> GCNT
> 
> TTNCCNGCNCCCCGCNCGTTCCTTTTAGNTTTCAAGTCTTTNCGCACCGTGCTTCCCCACGCGGAATGCTTTAATG
> ANTT
> 
> ACTTTCTNCCCCAAGGGAAATAGAAAACCCGNAATACCNAGTCTTNCTTGNTATACCNGCTNGGGCTTNCGGGGNA
> TCNA
> 
> ATNCCGTTTGCTCCNNCTTCCTTTCTCCNCNTNNCNNNTNGNTNCCNCCCNANAGGCNNCTTCCCCNATGGGATNC
> CTCN
> 
> CNTNCTCCNCTAGNNCCGNAAACTNGGTAAGAAGATNNTTTTTCTNNNAACNAGTAANTCTAAACGNACCACNNGG
> GATN
> 
> GGNAAAATAANTAATANANANAGGNACTAGACCAAGNTNNNGAGATNNTACCNAAAANNAANTTAGGGATNAGANA
> NAGN
> 
>>NTTTTANACTACT
>>
>>If you have any more information about this bacterium, please tell me,
>>I'm interested :)
>>
>>Regards,
>>Trond Erik
>>
>>
>>--
>>Trond Erik Vee Aune
>>Department of Biotechnology
>>Norwegian University of Science and Technology
>>Email: trondaun@biotech.ntnu.no
>>http://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen
>>
> 
> 
> 

Hate to say I told you so........but it just didn't sound like a gram negative to
me.

Bacillus will only sporulate under adverse conditions. If you're providing it with
breakfast, lunch, and dinner on a great plate, it has no need to form spores.

Judy Dilworth, M.T. (ASCP)
Microbiology (Clinical) 28 years

Trond Erik Vee Aune wrote:

> Yes, it was highly motile and swarmed in a very fascinating way. That's
> why I decided to identify it. I looked for spores in the microscope, but
> couldn't see any, so maybe the cells were in the wrong growth phase.
>
> Trond Erik
>
> >>>Anyway, I got very good match against Paenibacillus alvei. According to
> >>web-resources this genus do produce different types of antibiotics as
> >>well as being able to swarm in different ways
> >>(http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange thing
> >>is that it is not a gram negative bug as I thought after staining, but
> >>one of you microbiologists suggested that I was a Bacillus strain, so I
> >>guess we geneticists are much better at sequencing than staining ;)
> >>Anyway, I'm pretty pleased that I finally got an identification, but of
> >>course a little disappointed that it wasn't a new bacterium
>

JEDilworth wrote:
> Hate to say I told you so........but it just didn't sound like a gram negative to
> me.

Nah, don't feel bad about it. I'm just glad I learnt how to do 16s 
sequencing. Now I'm going to look for new bacteria, I just need to find 
some place where other people haven't been looking before.

Btw, I'm still working with the Ganges bacteria (Paenibacillus alvei). 
It seem to have a plasmid of less than 30kb, so I'm going to see if I 
can establish it in some other bacteria and see if some of the phenotype 
from the Paenibacillus is transferred to the new one, for instance 
antibiotics production. I need some practical work to do inbetween 
reading to exam :)

Trond Erik


> 
> Bacillus will only sporulate under adverse conditions. If you're providing it with
> breakfast, lunch, and dinner on a great plate, it has no need to form spores.
> 
> Judy Dilworth, M.T. (ASCP)
> Microbiology (Clinical) 28 years
> 
> Trond Erik Vee Aune wrote:
> 
> 
>>Yes, it was highly motile and swarmed in a very fascinating way. That's
>>why I decided to identify it. I looked for spores in the microscope, but
>>couldn't see any, so maybe the cells were in the wrong growth phase.
>>
>>Trond Erik
>>
>>
>>>>>Anyway, I got very good match against Paenibacillus alvei. According to
>>>>
>>>>web-resources this genus do produce different types of antibiotics as
>>>>well as being able to swarm in different ways
>>>>(http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange thing
>>>>is that it is not a gram negative bug as I thought after staining, but
>>>>one of you microbiologists suggested that I was a Bacillus strain, so I
>>>>guess we geneticists are much better at sequencing than staining ;)
>>>>Anyway, I'm pretty pleased that I finally got an identification, but of
>>>>course a little disappointed that it wasn't a new bacterium
>>>
> 

Well, you dont even have to look in places others havent looked... I 
think I mentioned this before, but anyhow - we found one growing as  a 
contaminant on a new agar plate in our cold room!



Trond Erik Vee Aune wrote:
> 
> 
> JEDilworth wrote:
> 
>> Hate to say I told you so........but it just didn't sound like a gram 
>> negative to
>> me.
> 
> 
> Nah, don't feel bad about it. I'm just glad I learnt how to do 16s 
> sequencing. Now I'm going to look for new bacteria, I just need to find 
> some place where other people haven't been looking before.
> 
> Btw, I'm still working with the Ganges bacteria (Paenibacillus alvei). 
> It seem to have a plasmid of less than 30kb, so I'm going to see if I 
> can establish it in some other bacteria and see if some of the phenotype 
> from the Paenibacillus is transferred to the new one, for instance 
> antibiotics production. I need some practical work to do inbetween 
> reading to exam :)
> 
> Trond Erik
> 
> 
>>
>> Bacillus will only sporulate under adverse conditions. If you're 
>> providing it with
>> breakfast, lunch, and dinner on a great plate, it has no need to form 
>> spores.
>>
>> Judy Dilworth, M.T. (ASCP)
>> Microbiology (Clinical) 28 years
>>
>> Trond Erik Vee Aune wrote:
>>
>>
>>> Yes, it was highly motile and swarmed in a very fascinating way. That's
>>> why I decided to identify it. I looked for spores in the microscope, but
>>> couldn't see any, so maybe the cells were in the wrong growth phase.
>>>
>>> Trond Erik
>>>
>>>
>>>>>> Anyway, I got very good match against Paenibacillus alvei. 
>>>>>> According to
>>>>>
>>>>>
>>>>> web-resources this genus do produce different types of antibiotics as
>>>>> well as being able to swarm in different ways
>>>>> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange 
>>>>> thing
>>>>> is that it is not a gram negative bug as I thought after staining, but
>>>>> one of you microbiologists suggested that I was a Bacillus strain, 
>>>>> so I
>>>>> guess we geneticists are much better at sequencing than staining ;)
>>>>> Anyway, I'm pretty pleased that I finally got an identification, 
>>>>> but of
>>>>> course a little disappointed that it wasn't a new bacterium
>>>>
>>>>
>>
>