Thread View: sci.bio.microbiology
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Started by Aldert Zomer
Mon, 19 Mar 2001 16:15
bacterial spores to vegetative cells
Author: Aldert Zomer
Date: Mon, 19 Mar 2001 16:15
Date: Mon, 19 Mar 2001 16:15
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Hi all, We're having some minor problems with the growth of vegetative cells from spores. We treat the spores with a heat shock, 85 oC for 5 minutes to induce growth but sometimes the spores just don't grow. (The spores in question are from clostridium beyerinckii and clostridium tyrobutyricum, found in cheese) Are there any other methods to induce the spores? -- Aldert Zomer
Re: bacterial spores to vegetative cells
Author: Jim and Annette
Date: Fri, 23 Mar 2001 11:27
Date: Fri, 23 Mar 2001 11:27
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Try reducing the temp in 5C increments. Maybe 85C is a little high for your strains. Jim
Re: bacterial spores to vegetative cells
Author: Martin Smith
Date: Thu, 05 Apr 2001 05:48
Date: Thu, 05 Apr 2001 05:48
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MAybe 85 degrees isint enough. I suggest a lower temperature for a longer interval of time (i.e. 50-60 gedrees for 30 mins). If this doesnt work, he spores are probably genetically defective or absent (I see no other solution). "Aldert Zomer" <zomeral@biol.rug.nl> wrote in message news:3AB630A4.7419C3B8@biol.rug.nl... > Hi all, > > We're having some minor problems with the growth of vegetative cells > from spores. We treat the spores with a heat shock, 85 oC for 5 minutes > to induce growth but sometimes the spores just don't grow. (The spores > in question are from clostridium beyerinckii and clostridium > tyrobutyricum, found in cheese) > > Are there any other methods to induce the spores? > > -- > Aldert Zomer
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