Thread View: sci.bio.microbiology
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Started by Joseph Passero
Fri, 26 Jan 2001 21:57
Looking for a Procedure to Collect Lymphocytes
Author: Joseph Passero
Date: Fri, 26 Jan 2001 21:57
Date: Fri, 26 Jan 2001 21:57
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I am looking for a procedure to collect 3ml of lymphocytes (not damaged) from fresh human blood. I have available a 15,000 rpm centrifuge (an Eppendorf 5412) and 2ml Eppendorf microcerterfuge tubes. Can some one recommend a site that may have a procedure or post/email me a procedure. Thank You Best Regards Joseph Passero mailto:jp@spacelab.net
Re: Looking for a Procedure to Collect Lymphocytes
Author: coxnews
Date: Sat, 27 Jan 2001 14:38
Date: Sat, 27 Jan 2001 14:38
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Joseph, try the following site http://www.the-scientist.com/yr1999/sept/cell_990913.pdf It lists the websites to several companies that provide cell separation media and methods. Of course the procedure you choose will be need to be based on why you are removing the lymphocytes (do you want just B cells, T cells, etc.or would it be allright if a few RBC's or other WBC's remain with the lymphocytes). We routinely use a solution called Lymphoprep to separate PBMC's from heparinized whole blood so that we can extract DNA from the PBMC's and perform PCR testing. Good Luck, Carl Johnson MT (ASCP) Joseph Passero <jp@spacelab.net> wrote in message news:3A71F2E7.CD832F54@spacelab.net... > > I am looking for a procedure to collect 3ml of lymphocytes (not damaged) from fresh human blood. > > I have available a 15,000 rpm centrifuge (an Eppendorf 5412) and 2ml Eppendorf microcerterfuge > tubes. > > Can some one recommend a site that may have a procedure or post/email me a procedure. > > Thank You > > Best Regards > > Joseph Passero > > mailto:jp@spacelab.net
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