Article View: sci.bio
Article #4589Re: General interest questions
From: Brian Smith-White
Date: Mon, 21 Feb 1994 23:34
Date: Mon, 21 Feb 1994 23:34
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2993 bytes
In Article <2ju5gv$g76@agate.berkeley.edu> "hcorbett@garnet.berkeley.edu ()" says: > Preamble: I am a TA for an introductory biology class (college level). To > spark my students' interest a little, I had them each write questions to > me, about anything in biology that interested them, and that at the > beginnning of each laboratory secFrom -6893434098533936382 X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 105a11,adbedc34cdad8faa X-Google-Attributes: gid105a11,public X-Google-Thread: 1119a6,adbedc34cdad8faa X-Google-Attributes: gid1119a6,public X-Google-ArrivalTime: 1994-02-18 09:22:39 PST Xref: gmd.de bionet.general:5287 bionet.molbio.methds-reagnts:9873 Newsgroups: bionet.general,bionet.molbio.methds-reagnts Path: gmd.de!newsserver.jvnc.net!howland.reston.ans.net!pipex!uunet!nih-csl!jowens.nci.nih.gov!jow From: Jim Owens <jow@helix.nih.gov> Subject: Re: Taq polymerase - what exonuclease activities? Message-ID: <1994Feb18.172239.21177@alw.nih.gov> X-Xxmessage-Id: <A98A63D3D4015621@jowens.nci.nih.gov> X-Xxdate: Fri, 18 Feb 94 12: 23:47 GMT Sender: postman@alw.nih.gov (AMDS Postmaster) Organization: NIH, Lab of Genetics X-Useragent: Version 1.1.3 References: <CLAF5n.Is6@massey.ac.nz> Date: Fri, 18 Feb 1994 17:22:39 GMT Lines: 20 In article <CLAF5n.Is6@massey.ac.nz> P. Hirst, PHirst@massey.ac.nz writes: >I'm trying find out exactly what exonuclease activities Taq polymerase >has, and which polymerase it is/ or which it's derived from. The >literature I've found so far is far from precise in its description, so >any information anyone has would be greatly appreciated. According to David Gelfand in Chapter 2 of PCR Technologies: Principles and Applications for DNA Amplification, Henry A. Erlich (ed), Stockton Press, 1989: "Purified 94 kDa Taq DNA polymerase does not contain an inherent 3'-5' exonuclease activity... It is not known if T. aquaticuys contain a separate 3'-5' exonuclease activity which may be associated with the polymerase in vivo.... Taq DNA polymerase has a DNA synthesis-dependent, strand replacement, 5'-3' exonuclease activity. (more details omitted)" (p.19) Hope this helps, Jim Owens
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 107c0a,8c24730de8411773 X-Google-Attributes: gid107c0a,public X-Google-Thread: 1119a6,8c24730de8411773 X-Google-Attributes: gid1119a6,public X-Google-ArrivalTime: 1994-02-21 15:34:00 PST Path: arc1-ca.pugleaf.net!gmd.de!newsserver.jvnc.net!news.edu.tw!news!nctuccca.edu.tw!howland.reston.ans.net!agate!msuinfo!preiss2.bch.msu.edu From: smithwhi@students.msu.edu (Brian Smith-White) Newsgroups: sci.bio,bionet.general Subject: Re: General interest questions Date: Mon, 21 Feb 1994 18:34 est Organization: Michigan State University Lines: 68 Distribution: world Message-ID: <19940221183430.smithwhi@preiss2.bch.msu.edu> References: <2ju5gv$g76@agate.berkeley.edu> NNTP-Posting-Host: preiss2.bch.msu.edu X-Newsreader: FTPNuz (DOS) v1.0