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Article #4589

Re: General interest questions

#4589
From: Brian Smith-White
Date: Mon, 21 Feb 1994 23:34
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In Article <2ju5gv$g76@agate.berkeley.edu> "hcorbett@garnet.berkeley.edu ()" says:
> Preamble: I am a TA for an introductory biology class (college level). To
> spark my students' interest a little, I had them each write questions to
> me, about anything in biology that interested them, and that at the 
> beginnning of each laboratory secFrom -6893434098533936382
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From: Jim Owens <jow@helix.nih.gov>
Subject: Re: Taq polymerase - what exonuclease activities?
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In article <CLAF5n.Is6@massey.ac.nz> P. Hirst, PHirst@massey.ac.nz writes:
>I'm trying find out exactly what exonuclease activities Taq polymerase
>has, and which polymerase it is/ or which it's derived from.  The
>literature I've found so far is far from precise in its description, so
>any information anyone has would be greatly appreciated.

According to David Gelfand in Chapter 2 of PCR Technologies: Principles
and Applications for DNA Amplification, Henry A. Erlich (ed), Stockton
Press, 1989:

"Purified 94 kDa Taq DNA polymerase does not contain an inherent 3'-5'
exonuclease activity...  It is not known if T. aquaticuys contain a
separate 3'-5' exonuclease activity which may be associated with the
polymerase in vivo....  Taq DNA polymerase has a DNA synthesis-dependent,
strand replacement, 5'-3' exonuclease activity.  (more details omitted)" 
(p.19)

Hope this helps,

Jim Owens
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From: smithwhi@students.msu.edu  (Brian Smith-White)
Newsgroups: sci.bio,bionet.general
Subject: Re: General interest questions
Date: Mon, 21 Feb 1994  18:34 est
Organization: Michigan State University
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