How do I extract free amino acids form leaf tissue?

Thread View: sci.chem.analytical

4 messages

[T] Back to Threads [V] Tree View [G] Group Articles

4 total messages Started by Tony Larson Fri, 29 Jun 2001 16:53

#76

Author: Tony Larson
Date: Fri, 29 Jun 2001 16:53

7 lines
1447 bytes

I'd like to know a method to extract free amino acids from small
(5-100 mg) amounts of plant leaf tissue.  These will than be
derivatized and analyzed by either HPLC or GC.  I would particularly
like to know the best way to clean up the extracts, to remove
background contaminants, in as small a working volume as possible
before derivatization.  This is because I will need to process a large
number of samples.

Re: How do I extract free amino acids form leaf tissue?

#77

Author: Lee Hunt
Date: Fri, 29 Jun 2001 18:55

12 lines
1910 bytes

I used to desalt with Sephadex G-25, although this can result in a
dilution

Tony Larson wrote:

> I'd like to know a method to extract free amino acids from small
> (5-100 mg) amounts of plant leaf tissue.  These will than be
> derivatized and analyzed by either HPLC or GC.  I would particularly
> like to know the best way to clean up the extracts, to remove
> background contaminants, in as small a working volume as possible
> before derivatization.  This is because I will need to process a large
> number of samples.

Re: How do I extract free amino acids form leaf tissue?

#78

Author: Lee Hunt
Date: Sun, 01 Jul 2001 20:16

9 lines
1563 bytes

Sorry thats if you want to do enzyme assays. You could concentrate by
evaporating the sample

Lee Hunt wrote:

> I used to desalt with Sephadex G-25, although this can result in a
> dilution
>
>

Re: How do I extract free amino acids form leaf tissue?

#79

Author: clarosa
Date: Fri, 06 Jul 2001 16:56

8 lines
1429 bytes

We always used methanolic (?) extraction.   Look of the paper by David
Rhodes in the late 1980 on proline and amino acid metabolism in Plant
Physiology for GC methods.

Tony Larson wrote:

> I'd like to know a method to extract free amino acids from small
> (5-100 mg) amounts of plant leaf tissue.  These will than be

Thread Navigation

This is a paginated view of messages in the thread with full content displayed inline.

Messages are displayed in chronological order, with the original post highlighted in green.

Use pagination controls to navigate through all messages in large threads.

Back to All Threads