🚀 go-pugleaf

recently,the biosynthesis of metabolites of arachidonic acid by  rabbit
washed platelet were investigated in our lab. The major products of
metabolism were 12-hydroxy-5,8,10-heptadecatrienoic acid(HHT) and
12-hydroxy-6,8,11,14-eicosatetraenoic acid(12-HETE). Analyse them on ODS
column by RPHPLC. There is reported that the amount of platelet must be
10^9/ml. First we mix the platelet with arachidonic acid, then
incubated for a while, the extract the products with ethyl acetate,
evaporate the ethyl acetate, and dissolve them in methanol, and then
apply them to the ODS-column. But we can't obtain anything. I seemed the
reaction has not occured. I don't know why. Can someone help me how to
control the reaction and how to isolate the products?

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