🚀 go-pugleaf

IIRC dry sephadex can be used instead of PEG. Not tried it myself tho.

Ian Mc

There is another technique called flash dialysis which involves side arm
flasks and vaccuum but I cant remember all the details. (28 and over the
hill)

Ian Mc



In article <628mds$lu4$1@agate.berkeley.edu>, lhom@nature.berkeley.edu
(Louis Hom) wrote:

> Uncle Al  <UncleAl0@ix.netcom.com> wrote:
> >
> >Poly(vinyl alcohol) is a solid.  If you have a liquid it is dissolved in
> >water.
> >
> >Why not hang the bag in a stream of dry air or pack it in a coffee can
> >with activated Drierite, silica gel, or molecular sieves?
> 
>         Aye, Uncle Al, poly(vinyl alcohol) is a solid.  We want it to soak
> up the water plus the small molecules (salt, buffer) that it contains,
> which is why a stream of dry air wouldn't work in my case.  Plus, we need
> to keep this protein cold.  So the idea would be to set the sample on a bed
> of dry PVA in the fridge.  Only thing is, I don't how good it would be at
> sucking away the buffer.  
> -- 
>
_______________________________________________________________________________
> Lou Hom >K '93                            
> lhom@nature.berkeley.edu                    
> http://www.ocf.berkeley.edu/~lhom