Re: A-level investigation into Urea/Urease reaction

Article View: sci.chem

Article #2298

#2298

From: William R. Penrose
Date: Tue, 26 Nov 1996 00:00

26 lines
1905 bytes

In article <MN$GEDAzIhmyEwQU@gertrood.demon.co.uk> Michael Johnson <mjohnson@gertrood.demon.co.uk> writes:

>I am currently planning an A-level chemistry investigation into the
>reaction kinetics of the urea/urease reaction. Can anyone please point
>me in the right direction in terms of methods of measuring rate,
>inhibitors to the reaction, how to extract urease, structure etc. Any
>websites, books or other additional innformation would also be handy.

This is a favorite expt for undergraduate biochem labs.  I suggest that you 
raid the library for lab manuals and look through them.  Urease is a nice 
rugged enzyme with a cheap and easy assay (the Conway dish) that can be used 
to demo most of the elementary principles of enzymology.

Bill

************************************************************
Bill Penrose, Sr. Scientist, Transducer Research, Inc.
   600 North Commons Drive, Suite 117
   Aurora, IL 60504
   630-978-8802, fax -8854, email wpenrose@interaccess.com
************************************************************
Purveyors of fine gas sensors and 
contract R&D to this and nearby galaxies.
************************************************************

Technical Details

Message-ID: <wpenrose.582.001930C0@interaccess.com>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

References: <MN$GEDAzIhmyEwQU@gertrood.demon.co.uk>

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: fd9c4,308c79670d4d1662
X-Google-Attributes: gidfd9c4,public
X-Google-Thread: f4886,308c79670d4d1662
X-Google-Attributes: gidf4886,public
X-Google-Thread: 10065c,308c79670d4d1662
X-Google-Attributes: gid10065c,public
From: wpenrose@interaccess.com (William R. Penrose)
Subject: Re: A-level investigation into Urea/Urease reaction
Date: 1996/11/26
Message-ID: <wpenrose.582.001930C0@interaccess.com>
X-Deja-AN: 200853294
distribution: world
references: <MN$GEDAzIhmyEwQU@gertrood.demon.co.uk>
organization: InterAccess,Chicagoland's Full Service Internet Provider
newsgroups: sci.chem,sci.bio.misc,bionet.molbio.proteins

Reply to Parent Message