Thread View: sci.med.immunology
4 messages
4 total messages
Started by Goran Pavlakovic
Wed, 27 Oct 1999 00:00
How to block/destroy Fc domain of an antibody?
Author: Goran Pavlakovic
Date: Wed, 27 Oct 1999 00:00
Date: Wed, 27 Oct 1999 00:00
14 lines
1078 bytes
1078 bytes
Hi there, does anybody have an idea how could I block/destroy Fc domain of an antibody (primary antibody) so that it would not be recognised by (otherwise) appropriate secondary antibody? Would incubation with Protein A do the trick? Thanx, Goran pavlakovic@gmx.net
Re: How to block/destroy Fc domain of an antibody?
Author: Tammy Ferguson
Date: Wed, 27 Oct 1999 00:00
Date: Wed, 27 Oct 1999 00:00
18 lines
1216 bytes
1216 bytes
Protein A will work for IgGs only and I'm not sure if it is subtype specific (IgG1, IgG2a,b, etc.) Goran Pavlakovic wrote: > Hi there, > > does anybody have an idea how could I block/destroy Fc domain of an > antibody (primary antibody) so that it would not be recognised by > (otherwise) appropriate secondary antibody? > > Would incubation with Protein A do the trick? > > Thanx, > > Goran > > pavlakovic@gmx.net
Re: How to block/destroy Fc domain of an antibody?
Author: Glen Tamura
Date: Wed, 27 Oct 1999 00:00
Date: Wed, 27 Oct 1999 00:00
25 lines
1583 bytes
1583 bytes
Protein A will definitely not do the trick. It binds only to a small portion of the Fc domain, and won't block a significant portion of your secondary antibody. What you need to do is make Fab fragments using pepsin. Protocols are available readily in textbooks. Glen Tamura On Wed, 27 Oct 1999, Goran Pavlakovic wrote: > > Hi there, > > does anybody have an idea how could I block/destroy Fc domain of an > antibody (primary antibody) so that it would not be recognised by > (otherwise) appropriate secondary antibody? > > Would incubation with Protein A do the trick? > > Thanx, > > Goran > > pavlakovic@gmx.net > >
Re: How to block/destroy Fc domain of an antibody?
Author: Goran Pavlakovic
Date: Wed, 27 Oct 1999 00:00
Date: Wed, 27 Oct 1999 00:00
33 lines
1719 bytes
1719 bytes
First of all, thanx to all that answered to my question so far (hope there will be more :-). And now a second question: What about biotinilation? Goran Glen Tamura schrieb: > Protein A will definitely not do the trick. It binds only to a small > portion of the Fc domain, and won't block a significant portion of your > secondary antibody. What you need to do is make Fab fragments using > pepsin. Protocols are available readily in textbooks. > > Glen Tamura > > On Wed, 27 Oct 1999, Goran Pavlakovic wrote: > > > > > Hi there, > > > > does anybody have an idea how could I block/destroy Fc domain of an > > antibody (primary antibody) so that it would not be recognised by > > (otherwise) appropriate secondary antibody? > > > > Would incubation with Protein A do the trick? > > > > Thanx, > > > > Goran > > > > pavlakovic@gmx.net > > > >
Thread Navigation
This is a paginated view of messages in the thread with full content displayed inline.
Messages are displayed in chronological order, with the original post highlighted in green.
Use pagination controls to navigate through all messages in large threads.
Back to All Threads