Thread View: sci.med.immunology
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Started by KAREN TILMANT
Mon, 13 Oct 1997 00:00
cytosol samples
Author: KAREN TILMANT
Date: Mon, 13 Oct 1997 00:00
Date: Mon, 13 Oct 1997 00:00
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Hello, Can anyone tell me what exactly are cytosol samples which are sometime used routinely for oestrogen-receptor? Thanks Karen
Re: cytosol samples
Author: Rick Greene
Date: Tue, 14 Oct 1997 00:00
Date: Tue, 14 Oct 1997 00:00
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In article <3442A625.49E6DF8D@rug.ac.be>, KAREN TILMANT <karen.tilmant@rug.ac.be> wrote: > Hello, > Can anyone tell me what exactly are cytosol samples which are sometime > used > routinely for oestrogen-receptor? > Thanks > Karen Hi Karen, My company formerly sold kits for the determination of estrogen and progestin receptors. A cytosol is prepared from biopsy tissue by homogenization of the weighed tissue in about 10 volumes of buffer containing a reducing agent to preserve the activity of the receptors, followed by ultracentrifugation in the cold at 105,000 x g for 30 minutes to remove the nuclei and other particulate material. A typical cytosol has a total protein content of 2 - 6 mg/mL. Let me know if you need any further details. Rick Greene
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