🚀 go-pugleaf

De Groote Dominic <dominic.degroote@rug.ac.be> wrote:

> Hello,
> 
> Does anybody know an antibody directed against p53 which crossreacts
> with canine tissue.
> 
> Thanks,
> 
> De Groote Dominic

Dr. Teifke, Inst. Vet.-Pathol. in Giessen, has some experience in this
field. 
Try: Teifke, J. P. & Lohr, C. V. (1996): Immunohistochemical detection
of P53 overexpression in paraffin wax-embedded squamous cell carcinomas
of cattle, horses, cats and dogs. Journal of Comparative Pathology 114:
205-210

Hope it helps!

-- 
dr jh walter           jhwpatho@zedat.fu-berlin.de
inst vet-pathol FU Berlin    tel +49-30-8108 25 47
strasse 518 nr. 15           fax +49-30-8108 25 22
D-14163 Berlin  Germany

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De Groote Dominic wrote:
> 
> Hello,
> 
> Does anybody know an antibody directed against p53 which crossreacts
> with canine tissue.
> 
> Thanks,
> 
> De Groote Dominic

Suggest you correspond with these folks.

-- 
Jeffrey Smoot  (jsmoot@cas.org)
614-447-3600 X3198
**************************************************************
Chemical Abstract Services http://www.cas.org
STN Easy http://stneasy.cas.org
ChemCemter http://www.ChemCenter.org
**************************************************************

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TI   p53 Tumor suppressor protein overexpression in osteogenic tumors of
     dogs
AU   Sagartz, J. E.; Bodley, W. L.; Gamblin, R. M.; Couto, C. G.; Tierney,
     L. A.; Capen, C. C.
CS   College Veterinary Medicine, Ohio State University, Columbus, OH, USA
SO   Vet. Pathol. (1996), 33(2), 213-21

AB   Alterations in the p53 tumor suppressor gene have been implicated in
     the genesis and/or progression of the majority of human cancers,
     including osteosarcoma.  Stabilization of the protein by mutation or
     interaction with other proteins prolongs its half-life, rendering it
     detectable by immunohistochem.  Osteosarcoma is the most common
     primary canine bone tumor and is characterized by frequent early
     metastases.  Multilobular tumors of bone involve primarily flat bones
     of the head and are low-grade malignancies with lower metastatic
     potential.  The objectives here were to det. the prevalence of p53
     protein overexpression in 106 osteogenic tumors of dogs using an
     indirect immunohistochem. method and to compare p53 overexpression
     between tumors with different clin. behavior.  A polyclonal p53
     antibody (CM-1) served as the primary antibody.  Tumors were scored
     based upon an est. of the percentage of tumor cells stained.
     Differences in the prevalence of overexpression were obsd. between
     osteosarcomas (72%) and multilobular tumors of bone (20%).
     Osteosarcomas of the appendicular skeleton had a higher prevalence of
     p53 overexpression (84%) than did osteosarcomas of the axial skeleton
     (56%).  Apparently, the p53 tumor suppressor protein is overexpressed
     in the majority of canine osteosarcomas.  The higher prevalence of
     overexpression in osteosarcomas vs. multilobular tumors of bone and
     in osteosarcomas of the appendicular skeleton vs. those of the axial
     skeleton suggests that alterations in p53 expression correlate with
     highly aggressive tumor behavior.

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In article <340D7608.5013@uic.edu> ronlab <ronlab@uic.edu> wrote:
> 
> Hi!
> 


Hi!

I am looking for plasmids that code for protein G (or its Ab binding fragments) or single chain Ab. A had 
an impression that a modified protein G was available through ATCC, but my resent search gave no results. 
I would appreciate any information on how and where such plasmids can be purchased.Generous gifts or 
offers of collaboration will be appreciated as well. Please reply to my e-mail address (U09577@uic.edu) or 
call me at (312)-4139198.

Eugene Kandel.
University of Illinois.
Department of Genetics.
U09577@uic.edu