Re: Stable mRNA's?

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From: Alexander Brands
Date: Wed, 09 Jun 1999 00:00

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> Sorry for not mentioning!
> Caco2-cells (colon carcinoma) treated with certain lipids.

Good to know...We work with Caco2 as well and found in western blots that Villin
is a useful internal standard. Should work for mRNA, too.

Alexander


>
> Jorgen
>
> In article <Jarmo.Schrader-0706991856230001@d177.genfys.slu.se>,
> Jarmo.Schrader@removethis.genfys.slu.se (Jarmo Schrader) wrote:
>
> > What cells? E.coli? Arabidopsis? Human? Drosophila?? Which tissue?
> >
> >
> > In article <jorgen.borrebak-0706991429450001@pkmac45.uio.no>,
> > jorgen.borrebak@basalmed.uio.no (J�rgen Borreb�k) wrote:
> >
> > > Hi!
> > >
> > > I'm doing expression studies and need to check the stability of my
> > > reference mRNA's when cells are subjected to treatment. Does anyone know
> > > any mRNA's that's known to be stably transcribed under different
> > > influences to the cells?
> > > (like G3PDH, beta-actin or other usual kontrols)
> > >
> > > Jorgen

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<jorgen.borrebak-0706991429450001@pkmac45.uio.no> <Jarmo.Schrader-0706991856230001@d177.genfys.slu.se> <jorgen.borrebak-0806991716050001@pkmac45.uio.no>

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From: Alexander Brands <alexander.brands@uni-tuebingen.de>
Subject: Re: Stable mRNA's?
Date: 1999/06/09
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References: <jorgen.borrebak-0706991429450001@pkmac45.uio.no> <Jarmo.Schrader-0706991856230001@d177.genfys.slu.se> <jorgen.borrebak-0806991716050001@pkmac45.uio.no>
To: "J�rgen Borreb�k" <jorgen.borrebak@basalmed.uio.no>
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