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ZOUGARI BEN ELKHAYAT  My Abdessadeq

Address for correspondance:
Laboratoire de Biotechnologie et Am�lioration des Plantes
Institut National Polytechnique,
Ecole Nationale Sup�rieure Agronomique de Toulouse
145, Avenue de Muret
F-31076 Toulouse Cedex
France
Tel: 33 5 61 34 12 64
Fax: 33 5 62 13 65 65
e-mail:sadeq04@infonie.fr

 As you can see in my Curriculum Vitae, i have my expertise and interests
in working on projects, such as plant breeding, diallels analysis,
statistics, plant-microbe interactions, molecular genetics and protein
biochemistry. Please find enclosed my Curriculum Vitae and the names of
tree Professors with whom i have worked.

With my best regards. references: C. PLANCHON, Professor head of the
Department of Biotechnology and Plant Breeding at l'Institut National
Polytechnique de Toulouse - Ecole Nationale Sup�rieure
AgronomiqueToulouse E-mail: planchon@ensat.fr Phone / Fax:  33 5.
62.13.65.65 M.T. ESQUERRE-TUGAYE, Professor Centre de Physiologie
V�g�tale ( Role of Lipoxygenase in plant-microorganism interactions)
Universit� Paul Sabatier de Toulouse - UMR5546 CNRS E-mail:
esquerre@cict.fr Phone / Fax:  T�l.  33 5.61.55.67.61 ROUGE, Professor
Universit� Paul Sabatier de Toulouse Laboratoire de Biologie Cellulaire
Email: rouge@cicit.fr T�l: 33 5 61 55 66 11



-----------------------------------
CURRICULUM VITAE
------------------------------------

1991-1996: PhD work in Plant Biotechnology at the University of Paul
Sabatier Toulouse III and l'Institut National Polytechnique de Toulouse -
Ecole Nationale Sup�rieure Agronomique under the guidance of Professor
Claude Planchon; head of the Department of Biotechnology and Plant
Breeding .
------------------------------------------------------------------------
1990 - 1991: End of studies Diploma (DEA) of Plant Biotechnology. Option:
Interaction Plant Micro-organism at the University of Paul Sabatier
Toulouse France
------------------------------------------------------------------------
1989 - 1990: Master of Cellular Biology. Option: Microbiology at the
University of Paul Sabatier Toulouse, France
------------------------------------------------------------------------
1988 - 1989: Licence Diploma of Biochemistry at the University of Paul
Sabatier Toulouse, France
------------------------------------------------------------------------
1986 - 1988: General University studies: Diploma of Biology at the
University of Cadi Ayyad, Facult� des sciences Marrakech, Morocco
------------------------------------------------------------------------
June 1986: Bachelorship of Natural Sciences, Marrakech, Morocco
------------------------------------------------------------------------


SPECIALIZATIONS :

� Genetic improvement of soybean seed quality:	Genetic determinism study
of the expression of soluble proteins and lipoxygenases seed contents
with a complete diallel between five genotypes, in natural conditions and
in pots. � RAPD (PCR) in soybean genotype � diallel Analyse using
Griffing et Hayman methods � Statistics analysis: PCSM, Systat, Statitcf
and Sigma Plot. � Plant physiology: relations (carbon) C-N (nitrogen),
N-mobilization analysis during seed filling peroid � Biochemistry,
Enzymology et Immunology: � isolation and purification of  seed protein
by affinity Chromatography on Sephadex G100 �  determination  of
nitrogenase activity of soybean nodule using the Acetyl�ne Reduction
Assay by gaz Chromatography (DELSI DI 200) � determination of soybean
seed lipoxygenase activity polarographically using an Oxygraph Hansatech
DWI. � spectroscopy Analysis:  UV direct Spectroscopy � UV Difference
Spectroscopy � Polyacrylamide gel electophoresis using the Phast-system
(Pharmacia) � Proteins determinations:	a- de Kjeldahl Method  b-
colorimetric Method BCA (Pierce) � hemagglutination Technique:
determination of hemagglutination activity on standart micro-titration
plates � Indirect ELISA (Enzyme Linked Immunosorbent Assay) measurement
on standart micro-plates using either rabbit polyclonal antibodies
against PsA or monoclonal antibody (6,F8) made against the Lathyrus
odoratus lectin.


REFERENCES

1) ZOUGARI, A., GUY, S., and PLANCHON, C. 1995. Genotypic lipoxygenase
variation in soybean seeds and response to nitrogen nutrition. Plant
Breeding 114, 313-316. 2) ZOUGARI, A., P., CH�NE., H. MAZARGUIL., and P.
ROUG�. 1993. Unfolding of pea lectin in the presence of guanidium
chloride and urea. Lectins: Biology, Biochemistry, Clinical Biochemistry.
[Ed] Textop. p143-150. 3) ZOUGARI, A., SUC, S., and PLANCHON, C. 1995.
Am�lioration de la qualit� de la graine (improvement of soybean seed
quality):  � voies g�n�tiques et agronomiques: Param�tres de la qualit�
de la graine. Groupe pluridisciplinaire de recherche sur le soja,
Auzeville, F�v. 1992, 2-6. 4) ZOUGARI, A. 1995. Am�lioration de la
qualit� de la graine Communication orale. Groupe pluridisciplinaire de
recherche sur le soja, Auzeville, F�v. 1992. 5) ZOUGARI, A., PLANCHON, C
 ., and ECOCHARD, R. 1996. Inheritence of protein content and lipoxygenase
activity in soybean seed (Glycine max (L.) merr.) in cours of writing. 6)
ZOUGARI, A. 1990. D�naturation de la lectine de pois: approche
structurale et biologique. D.E.A U.P.S.Toulouse , 23 p 7) ZOUGARI, A.
1996. Genetic improvement of soybean seed quality (Glycine max (L. )
merr. ): lipoxygenases, and proteins and response to nitrogen nutrition
'. PhD thesis  INP,119 p.


 -------------------------------------
SUMMARY OF PhD THESIS
-------------------------------------

TITLE: Genetic improvement of soybean seed quality (Glycine max (L. )
merr. ): lipoxygenases, and proteins and response to nitrogen nutrition.
------------------------------------------------------------------------

SUMMARIZES: The use of soybean seed in human foods can be favored by a
reduction of the lipoxygenase content. This investigation aim to analyze
the variability and the genetic determinism of lipoxygenase and proteins
seed contents. The study of lipoxygenase activity, determined
polarographically on seeds taken from plants grown under controlled
conditions with a mineral or symbiotique nitrogen feeding, shows a large
genotypique variability for lipoxygenases contents, enhanced by high
dinitrogen fixation. In these same conditions of culture, during the seed
filling period, lipoxygenases accumulation appears massive until the
physiological maturity stage (R7) and is followed by a partial
degradation. The lipoxygenases may play a role in the temporary storage
of nitrogen. Finally a genetic study is realized in natural conditions
and in pots, with a complete diallel between five genotypes,
representative of a great variability for soluble proteins and
lipoxygenases seed contents. Hybrids F1 show a strong effect of heterosis
for soluble proteins, lipoxygenase 1 and total lipoxygenases contents.
For these last, hybrids show a strong homeostatique behavior with weaker
contents than those of the parents. Though, values of contents in soluble
proteins indicate a positive overdominance as well as a great sensitivity
to environnementales variations. Genetic determinism of the expression of
soluble proteins and lipoxygenases contents appears complex and linked to
significants additive, non-additive and reciprocal effects. Thus, the
reduction of lipoxygenases contents and the improvement of proteins
contents can be considered on a genetic context implying the nuclear and
cytoplasmique genomes together with more efficent dinitrogen fixation,
with respect of dinitrogen fixation.

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