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Article #813

Re: LAL testing

#813
From: rboosey Richard Boosey
Date: Tue, 04 Feb 1997 00:00
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I assume that you are interviewing for a position in a microbiology
laboratory.  The LAL test (Limulus amino lysate (sp?)) is a test for
pyrogens in products which has for the most part replaced the old
rabbit with thermocouple test (probably cheaper and a whole lot less
wear-and-tear on the rabbits, especially since those failing the  test
may have gone home as someone's dinner).  

The test  uses a constituent from the blood of the horseshow crab.
The initial commercial development was at Mallinckrodt by Don Mills
who worked at a bench behind mine at the time. The assay is based on
the congealing of the LAL with the product being tested. (Obviously, I
am a chemist, not a microbiologist). Sugggest you look at any number
of lab supply catalogs and give them a call for tear sheets, etc on
the test.  The initial tests were very tricky and operator dependent
and I don't know whether that has changed.

Good luck on the interview.

Rich


Rebecca Thron <thron@clam.rutgers.edu> wrote:

>Help!!

>I have a job interview that requires LAL testing and I have no idea what
>it is!  If anyone can help please email or post me.

>Thanks so much.

>BecRU97@Juno.com
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From: rboosey@aol.com@primenet.com (Richard Boosey)
Subject: Re: LAL testing
Date: 1997/02/04
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