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Article #14Photographing bacteria in a Gram's stain
From: First of Three of Six
Date: Sun, 10 Jul 2005 10:11
Date: Sun, 10 Jul 2005 10:11
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2105 bytes
Hi can anyone share tips and tricks on capturing images of bacteria stained by Gram's method. I'm using an Olympus BX51 with a DP70 and capturing the images using Olysia BioReport. While I'm finding the process excellent for larger organisms via a 40 objective, when using oil 100 focusing and sharpness have suffered. Everything looks good on the screen, but then I transfer the image (saved as tif and usually around 36 MB) and attempt some manipulation with Photoshop to resize to 96 dpi and save as jpg (10/10 quality) the picture degrades a little. Thanks in advance. I'm mostly keen to get any help on the best focussing techniques people use.
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X-Google-Thread: 106237,39b742f934e8b7b0,start X-Google-Thread: f6a65,39b742f934e8b7b0,start X-Google-Thread: 104764,39b742f934e8b7b0,start X-Google-Thread: 10813c,39b742f934e8b7b0,start X-Google-Thread: f60cd,39b742f934e8b7b0,start X-Google-Attributes: gid106237,gidf6a65,gid104764,gid10813c,gidf60cd,public X-Google-Language: ENGLISH,ASCII-7-bit Path: arc1-ca.pugleaf.net!g2news1.google.com!news3.google.com!news.glorb.com!in.100proofnews.com!in.100proofnews.com!news.cse.sc.edu!ken-transit.news.telstra.net!lon-in.news.telstra.net!news.telstra.net!news-server.bigpond.net.au!53ab2750!not-for-mail Reply-To: "First of Three of Six" <georgewbush@washington.com> From: "First of Three of Six" <firstofthreeofsix@hotmail.com> Newsgroups: sci.techniques.microscopy,bionet.microbiology,sci.bio.microbiology,sci.med.laboratory,sci.med.pathology Subject: Photographing bacteria in a Gram's stain Lines: 14 Organization: The Federation X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 6.00.2900.2180 X-RFC2646: Format=Flowed; Original X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 Message-ID: <Dv6Ae.36608$oJ.11643@news-server.bigpond.net.au> Date: Sun, 10 Jul 2005 10:11:47 GMT NNTP-Posting-Host: 60.231.211.28 X-Complaints-To: abuse@bigpond.net.au X-Trace: news-server.bigpond.net.au 1120990307 60.231.211.28 (Sun, 10 Jul 2005 20:11:47 EST) NNTP-Posting-Date: Sun, 10 Jul 2005 20:11:47 EST