Article View: sci.techniques.microscopy
Article #19Re: Photographing bacteria in a Gram's stain
From: orbii
Date: Thu, 14 Jul 2005 10:51
Date: Thu, 14 Jul 2005 10:51
25 lines
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2775 bytes
"First of Three of Six" <firstofthreeofsix@hotmail.com> wrote in message news:Dv6Ae.36608$oJ.11643@news-server.bigpond.net.au... > Hi can anyone share tips and tricks on capturing images of bacteria > stained by Gram's method. > I'm using an Olympus BX51 with a DP70 and capturing the images using > Olysia BioReport. > While I'm finding the process excellent for larger organisms via a 40 > objective, when using oil 100 focusing and sharpness have suffered. > Everything looks good on the screen, but then I transfer the image (saved > as tif and usually around 36 MB) and attempt some manipulation with > Photoshop to resize to 96 dpi and save as jpg (10/10 quality) the picture > degrades a little. > Thanks in advance. I'm mostly keen to get any help on the best focussing > techniques people use. you might want to check a few things in photoshop if u having grungy resizing issues. look in preferences, and make sure that IMAGE INTERPOLATION is set to no less then BICUBIC (SMOOTHER). once that's set, now rezise it again and see. another thing too is that you might want to view your files at 100% resolution, or u'll confuse yourself. when you saving jpg also, u can do 300dpi, i'd suggest doing 300dpi. works good both for prints and also if need be easy to convert to 72 web size.
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X-Google-Thread: 106237,39b742f934e8b7b0 X-Google-Thread: f6a65,39b742f934e8b7b0 X-Google-Thread: 104764,39b742f934e8b7b0 X-Google-Thread: 10813c,39b742f934e8b7b0 X-Google-Thread: f60cd,39b742f934e8b7b0 X-Google-Attributes: gid106237,gidf6a65,gid104764,gid10813c,gidf60cd,public X-Google-Language: ENGLISH,ASCII-7-bit Path: arc1-ca.pugleaf.net!g2news1.google.com!news4.google.com!news.glorb.com!news-feed01.roc.ny.frontiernet.net!nntp.frontiernet.net!newscon06.news.prodigy.com!prodigy.net!news-east.rr.com!news.rr.com!news-server.columbus.rr.com!cyclone2.kc.rr.com!news2.kc.rr.com!tornado.socal.rr.com.POSTED!53ab2750!not-for-mail From: "orbii" <orbii@hotmail.com> Newsgroups: sci.techniques.microscopy,bionet.microbiology,sci.bio.microbiology,sci.med.laboratory,sci.med.pathology References: <Dv6Ae.36608$oJ.11643@news-server.bigpond.net.au> Subject: Re: Photographing bacteria in a Gram's stain Lines: 28 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 6.00.2900.2527 X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2527 X-RFC2646: Format=Flowed; Original Message-ID: <6trBe.7425$rF5.7021@tornado.socal.rr.com> Date: Thu, 14 Jul 2005 10:51:46 GMT NNTP-Posting-Host: 66.8.166.151 X-Complaints-To: abuse@rr.com X-Trace: tornado.socal.rr.com 1121338306 66.8.166.151 (Thu, 14 Jul 2005 03:51:46 PDT) NNTP-Posting-Date: Thu, 14 Jul 2005 03:51:46 PDT Organization: Road Runner High Speed Online http://www.rr.com