Article View: sci.techniques.microscopy
Article #57Re: GIM, new technology
From: Joachim Wesner
Date: Sun, 19 Oct 1997 00:00
Date: Sun, 19 Oct 1997 00:00
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2251 bytes
Eyal Peleg wrote: > > Meir Ben-Levy <gimsyst@mail.netvision.net.il> wrote in article > <NEWTNews.876440189.23525.gimsyst@dialup.netvision.net.il>... > > > > Dear Reader, > > We are looking for partners to develop a new Confocal technology. > > > > Our group develops a new technology for Confocal Imaging by the > projection of > > sinusoidal pattern with incoherent illumination, rather than the focused > laser > > beam in the LSM technology. > <Snip> > The lateral (x, y) resolution: > The sine illumination shifts down the spatial frequencies of the object, > so the optical microscope observe lower spatial frequencies. That way, it > is possible to "sneak in" high frequencies that normally cannot be observed > with optical microscopes. > <Snip> *ANY* resolution improvement in scanning microscopy can be explained by the "mixing down" of higher spatial frequencies by the illumination function, incoherent or not. So, not a really new idea. If you use a ring pupil in the illumination optics, you can get very near to your concept (only the highest spatial frequencies in the beam), while still keeping (rotational) symmetry. > This is equivalent to having a numerical aperture of NA=1 in air or NA=1.5 > in oil objectives. > Depends on what kind of optics you use to actually generate the illumination function. If you are limited to NA 0.95 / NA 1.4 there, you will also not really get to equivalent 1.0/1.5 in the end (besides the difference being marginal). Joachim
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 106237,ec080b84fbc749c X-Google-Attributes: gid106237,public X-Google-Thread: 114d69,ec080b84fbc749c X-Google-Attributes: gid114d69,public From: Joachim Wesner <joachim.wesner@frankfurt.netsurf.de> Subject: Re: GIM, new technology Date: 1997/10/19 Message-ID: <344A2DCF.77F75628@frankfurt.netsurf.de> X-Deja-AN: 281786984 References: <NEWTNews.876440189.23525.gimsyst@dialup.netvision.net.il> <01bcdc23$ca5893e0$3d0b5ac2@default> X-Complaints-To: abuse@omnilink.net X-Trace: gazette.omnilink.net 877276744 7519 (None) 194.64.181.171 Organization: OMNILINK --- your link to the net Newsgroups: sci.techniques.microscopy,bit.listserv.confocal