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Article #57

Re: GIM, new technology

#57
From: Joachim Wesner
Date: Sun, 19 Oct 1997 00:00
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Eyal Peleg wrote:
> 
> Meir Ben-Levy <gimsyst@mail.netvision.net.il> wrote in article
> <NEWTNews.876440189.23525.gimsyst@dialup.netvision.net.il>...
> >
> > Dear Reader,
> > We are looking for partners to develop a new Confocal technology.
> >
> > Our group develops a new technology for Confocal Imaging by the
> projection of
> > sinusoidal pattern with incoherent illumination, rather than the focused
> laser
> > beam in the LSM technology.
>

<Snip>

>  The lateral (x, y) resolution:
>  The sine illumination shifts down the spatial frequencies of the object,
> so the optical microscope observe lower spatial frequencies. That way, it
> is possible to "sneak in" high frequencies that normally cannot be observed
> with optical microscopes.
> 

<Snip>

*ANY* resolution improvement in scanning microscopy can be explained
by the "mixing down" of higher spatial frequencies by the illumination
function, incoherent or not. So, not a really new idea. If you use a
ring pupil in the illumination optics, you can get very near to your
concept (only the highest spatial frequencies in the beam), while still
keeping (rotational) symmetry.

> This is equivalent to having a numerical aperture  of NA=1 in air or NA=1.5
> in oil objectives.
> 
Depends on what kind of optics you use to actually generate the
illumination function. If you are limited to NA 0.95 / NA 1.4 there, you
will also not really get to equivalent 1.0/1.5 in the end (besides the
difference being marginal). 


Joachim
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From: Joachim Wesner <joachim.wesner@frankfurt.netsurf.de>
Subject: Re: GIM, new technology
Date: 1997/10/19
Message-ID: <344A2DCF.77F75628@frankfurt.netsurf.de>
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