🚀 go-pugleaf

In article <4516pc$k6@dwst13.edvz.sbg.ac.at>, Anton Hutticher
<huttich@edvz.sbg.ac.at> wrote:

> 
> We compared NOVEX precast PAGE gels with our gels. The 
> resolution was quite good but not as good as our own specially
> crafted gradient gels. However, they were very easy to setup 
> and use. Several people switched to NOVEX, other stayed with 
> their own gels. If you do PAGE only sporadically, precasts are
> probably a good solution.
> Take care if you want to do western blots with precasts. Those
> from NOVEX we used could not stand the blotting conditions we 
> used ( 1 l  MeOH, 4,1 g NaHCO3, 1,6 g Na2CO3 ), 40 V, 3 h on
> Biorad Mini. They fragmented during the run and the fragments
> were very hard to peel off the nitrocellulose. We have not tested
> them under any other conditions! Maybe you find blotting conditions
> which work for them, but test them beforehand!
> 
> Anton Hutticher
> (huttich@edvz.sbg.ac.at)
> (Uni Salzburg / Austria) 

I use Novex precast gels for westerns. They run great in Tris/Glycine/SDS
and also in CAPS blotting buffers (200 mA for 2 hrs). I also occasionally
use precast and pre-EtBr-stained agarose gels from FMC Bioproducts. They
are also ok. 

Tomas Drgon

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