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Article #1416

PCR at 3000 masl

#1416
From: Andre van 't Slot
Date: Mon, 29 May 1995 00:00
29 lines
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	Finally the solution to our PCR problem. It has been a long time
since we received the last suggestions for this problem and we hope that
you can still remember our questions about PCR at 3050 meters above sea
level. Water boils at 89 C and we were afraid that we would not be able
to heat up the tubes to 94 C. 
	We are very grateful to all of you who thought about it and gave
us suggestions. Fortunately, the capped tubes consisting sample and
mineral oil did not pop open and reached 94 C without problem. So far we
have not been able to find out if the high altitude causes other problems.


			With friendly greetings,
				
					Greg, Andre and Mariu.
					CIP-Quito.
--
	Andre van 't Slot
	Centro Internacional de la Papa
	Casilla 17-16-129-CEQ
	Quito, Ecuador
	Telf. +593-2-690990, Internet: VANTSLOT@cip.org.ec

--
	Andre van 't Slot
	Centro Internacional de la Papa
	Casilla 17-16-129-CEQ
	Quito, Ecuador
	Telf. +593-2-690990, Internet: VANTSLOT@cip.org.ec
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From: vantslot@MAIL.CIP.ORG.EC (Andre van 't Slot)
Subject: PCR at 3000 masl
Date: 1995/05/29
Message-ID: <9505291035.AA02942@mail.cip.org.ec>
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newsgroups: bionet.diagnostics