Article View: bionet.diagnostics
Article #1416PCR at 3000 masl
From: Andre van 't Slot
Date: Mon, 29 May 1995 00:00
Date: Mon, 29 May 1995 00:00
29 lines
1446 bytes
1446 bytes
Finally the solution to our PCR problem. It has been a long time since we received the last suggestions for this problem and we hope that you can still remember our questions about PCR at 3050 meters above sea level. Water boils at 89 C and we were afraid that we would not be able to heat up the tubes to 94 C. We are very grateful to all of you who thought about it and gave us suggestions. Fortunately, the capped tubes consisting sample and mineral oil did not pop open and reached 94 C without problem. So far we have not been able to find out if the high altitude causes other problems. With friendly greetings, Greg, Andre and Mariu. CIP-Quito. -- Andre van 't Slot Centro Internacional de la Papa Casilla 17-16-129-CEQ Quito, Ecuador Telf. +593-2-690990, Internet: VANTSLOT@cip.org.ec -- Andre van 't Slot Centro Internacional de la Papa Casilla 17-16-129-CEQ Quito, Ecuador Telf. +593-2-690990, Internet: VANTSLOT@cip.org.ec
Technical Details
Message-ID:
<9505291035.AA02942@mail.cip.org.ec>Path:
arc1-ca.pugleaf.net!unknown!not-for-mailShow Full Headers
Complete Article Headers
X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,b5c088d1f13791c2,start X-Google-Attributes: gid10eaff,public From: vantslot@MAIL.CIP.ORG.EC (Andre van 't Slot) Subject: PCR at 3000 masl Date: 1995/05/29 Message-ID: <9505291035.AA02942@mail.cip.org.ec> X-Deja-AN: 103526479 distribution: world sender: daemon@net.bio.net organization: BIOSCI International Newsgroups for Molecular Biology newsgroups: bionet.diagnostics