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Article #1466Re: PCR-related techniques for detecting food born pathogens.
From: Kevin O'Donnell
Date: Tue, 04 Apr 1995 00:00
Date: Tue, 04 Apr 1995 00:00
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How about devising a system based on detecting RNA? This is degraded faster than DNA, so you'd have less chance of false positives based on nucleic acids from dead cells. Another alternative approach would be to follow up any PCR positives with traditional plating-out, with a positive result in both required for confirmation of the presence of the pathogen in food. There would still be an advantage in using PCR because it would screen out the negative samples. No references for either of these - just a couple of ideas that occured to me over my mid-morning cuppa:-) Kevin O'Donnell Scottish Agricultural Science Agency Edinburgh Scotland
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,74bb552ffba42fa8 X-Google-Attributes: gid10eaff,public From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Re: PCR-related techniques for detecting food born pathogens. Date: 1995/04/04 Message-ID: <D6I8yB.75I@jura.sasa.gov.uk> X-Deja-AN: 100841573 sender: news@jura.sasa.gov.uk (Usenet) references: <01HOWV77I5Z60007RI@GW.AGR.CA> organization: Scottish Agricultural Science Agency newsgroups: bionet.diagnostics