Use of Value of TCID50 result

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1 total messages Started by masud Wed, 16 May 2012 15:57

#331

Author: masud
Date: Wed, 16 May 2012 15:57

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I have measured TCID50 value of virus: 1.6X10^4/ml. Now how do I use
the value in the real infection assay? If the infection fianl volume
is 100 microliter, for 1 TCID50 (of above value) or 100 TCID50 (of the
same virus stock) what volume we need to infect? Does the cell number
in 100 microliter is a concern or not? If any body can discuss.
Thanks.

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