Re: Fluorescence quantification

Article View: bit.listserv.confocal

Article #125

Back to bit.listserv.confocal Previous Next

#125

From: Kris.Vissenberg
Date: Thu, 15 Jun 2000 00:00

36 lines
2250 bytes

Dear Dr. Berg,

At the lab we also quantify DNA in single cells of tobacco that undergo
endoreduplication. You can find details on how to do it in

Valente, P, Tao, W and Verbelen, J-P (1998) Auxins and cytokinins control
DNA endoreduplication an deduplication in single cells of tobacco. PLant
Science 134, 207-215.

We do not use DAPI but propidium iodide (and RNase treatment) instead. 
This makes it possible to quantify the amount of fluorescence with a
confocal microscope not equipped with a UV-laser. 

Hope this helps,


best wishes,


Kris


---------------------------------------------------------------------------
Kris Vissenberg                       _/      _/    _/     _/_/_/_/
PhD-Student                          _/      _/          _/      _/
Dept.Biology (C 0.24)               _/      _/    _/    _/      _/
Plant Physiology / Morphology      _/      _/    _/    _/      _/
University of Antwerp (UIA)       _/      _/    _/    _/_/_/_/_/
Universiteitsplein 1             _/      _/    _/    _/      _/
B - 2610 Wilrijk (Antwerpen)    _/      _/    _/    _/      _/
Belgium                          _/_/_/_/    _/    _/      _/

Phone :+32-3-820.22.58
Fax n°:+32-3-820.22.71
E-mail:vissenbe@uia.ua.ac.be
---------------------------------------------------------------------------

Technical Details

Message-ID: <Pine.OSF.4.21.0006150943130.10829-100000@hgins.uia.ac.be>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

References: <Pine.SOL.4.10.10006141815190.15594-100000@srv1.mail.uni-kiel.de>

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII
X-Google-Thread: 114d69,b2400f47d4c52d86,start
X-Google-Attributes: gid114d69,public
From: "Kris.Vissenberg" <vissenbe@uia.ua.ac.be>
Subject: Re: Fluorescence quantification
Date: 2000/06/15
Message-ID: <Pine.OSF.4.21.0006150943130.10829-100000@hgins.uia.ac.be>
X-Deja-AN: 634783418
Content-Transfer-Encoding: QUOTED-PRINTABLE
References: <Pine.SOL.4.10.10006141815190.15594-100000@srv1.mail.uni-kiel.de>
X-Original-NNTP-Posting-Host: hgins.uia.ac.be
To: Confocal Microscopy List <CONFOCAL@listserv.acsu.buffalo.edu>
X-Sender: vissenbe@hgins.uia.ac.be
Content-Type: TEXT/PLAIN; charset=ISO-8859-1
X-Complaints-To: abuse@belnet.be
X-Trace: naxos.belnet.be 961055392 23408 143.169.254.1 (15 Jun 2000 07:49:52 GMT)
Organization: University of Antwerp - UIA
Mime-Version: 1.0
NNTP-Posting-Date: 15 Jun 2000 07:49:52 GMT
Newsgroups: bit.listserv.confocal

Reply to Parent Message