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Article #134BSL images and deconvolution
From: Bartek Rajwa
Date: Wed, 23 Aug 2000 00:00
Date: Wed, 23 Aug 2000 00:00
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1733 bytes
I have a question concerning deconvolution. I am wondering if it is possible and reasonable to deconvolve confocal (or partially confocal) images obtained in backscattered light mode. I assume that in such a case PSF function would be dependent on size of backscattering particles present in the sample and pace of their Brownian movement. Am I correct? If so, how can I determine a model PSF which might be used later for a deconvolution? Surely I cannot obtain it experimentaly relying on an image of a single reflective bead since BSL impulse response function depends strongly on the neighborhood of the bead observed. What about blind deconvolution? Any ideas? Bartek Rajwa -- bartek@mol.uj.edu.pl ``Does anybody else find irony in the fact that Bill Gates says he's trying to write a stable OS, and Linus claims he's trying to take over the world?'' Anonymous
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 114d69,56ca149633a3ae9e,start X-Google-Attributes: gid114d69,public From: Bartek Rajwa <rajwa@helios.mol.uj.edu.pl> Subject: BSL images and deconvolution Date: 2000/08/23 Message-ID: <Pine.LNX.4.21.0008230957430.10408-100000@helios.mol.uj.edu.pl> X-Deja-AN: 661461787 References: <DFBD5F00372AD311905900A0C9ED07A11BC756@bioserver1.biology.usu.edu> <p04320402b5c77d5fe39b@[144.92.238.207]> To: Confocal Microscopy List <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU> Content-Type: TEXT/PLAIN; charset=US-ASCII X-Complaints-To: news@cyf-kr.edu.pl X-Trace: info.cyf-kr.edu.pl 967020163 4147 149.156.87.70 (23 Aug 2000 08:42:43 GMT) Organization: Academic Computer Center CYFRONET AGH Mime-Version: 1.0 NNTP-Posting-Date: 23 Aug 2000 08:42:43 GMT Newsgroups: bit.listserv.confocal