🚀 go-pugleaf

On Mon, 11 Sep 2000, Barbara Goettgens wrote:
> It seems to be a common problem of inverted microscopes that the nosepiece
> (with the objectives) has some drift.  He told me a number of things to do
> to check out whether its that or the stage. Haven't done that yet.
> The most important thing is to wait at least 15 minutes after you put your
> sample on togive the objective and the oil time to get into some stable
> state.  Does the shape look different in every single image or just after
> 30 minutes?
> Is that the microscope in pharmacy? The 2-photon?
> 
> Barbara
> 

Barbara,
this is very interesting! We have drift problems at our inverted Zeiss
LSM410, although most apparent to us in the x/y direction. The Zeiss
serviceman said this is normal, but didn't tell us how to find the cause.
Could you tell me how to find out if it is the stage or
the nosepiece?
Leaving the sample on the stage for some time definitely helps, but once
you move the sample or the focus by some amount, the drift comes back.

Joachim

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  Joachim Walter, Dipl. Phys.
  Institut für Anthropologie und Humangenetik der LMU München
  AG Cremer
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  D-80333 München               Tel. +49 - 89-2180-6713
  Germany                       Fax               -6719

  J.Walter@lrz.uni-muenchen.de
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