🚀 go-pugleaf

Tom,

I have used an antibody generated against the coat protein of a plant
virus, that worked beautifully for western, LM immunochemistry, and
immunoEM. LM labeling was done using paraffin embedded samples and the EM
labeling work was done on-section.

> ----- Original Message -----
> From: Tom Phillips <PhillipsT@MISSOURI.EDU>
> Newsgroups: bit.listserv.confocal
> To: <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU>
> Sent: Tuesday, September 19, 2000 12:16 AM
> Subject: % of antibodies that work in immuno
> 
> 
> > Lots of antibodies don't work at LM immunocytochemistry and even more
> > don't work at the EM level.  I would like to make an informal survey.
> > If you respond to me or the list, I will post a summary to the list.
> >
> > Here are the questions:
> >
> > Have you generated antibodies that work in some method (ELISA,
> > Westerns, immunoprecipitation) but not LM immunocytochemistry?
> >
> > Were they monoclonals or polyclonals?
> >
> > What percentage of the antibodies you have made work at the LM level
> > but not for EM immunocytochemistry?
> >
> > If you have made antibodies against synthetic peptides, what
> > percentage work in either LM or EM?
> >
> > Thanks, Tom
> >
> > --
> > Thomas E. Phillips, Ph.D.
> > Associate Professor of Biological Sciences
> > Director, Molecular Cytology Core Facility
> >
> > 3 Tucker Hall
> > Division of Biological Sciences
> > University of Missouri
> > Columbia, MO 65211-7400
> > (573)-882-4712 (voice)
> > (573)-882-0123 (fax)
>