🚀 go-pugleaf

Related questions:
1.  Does anyone know the refractive index of Citifluor?  (it's a
glycerol/phosphate buffer solution)
2.  If I could locate a refractometer, the ones I remember using have a white
light source.  Since refractive index depends on wavelength, how would I convert
my measurement to that at the proper wavelength?
3.  In general, does adding solutes INCREASE the refractive index of the solvent?
(i.e. for Citifluor, I would at least expect n to be larger than that of glycerol)

Thanks in advance-
Sarah

"Colin S. Izzard" wrote:

> The absolute refractive index for pure water  is 1.33335 at 20 degrees
> C for sodium light, lambda = 5893 Angstrom units  - see The Rubber
> Handbook.
> By definition, the refractive index of vacuum is 1.000.
> So where was the value of 1.025 for sea water published?
>
> Colin Izzard
>
> > Date:          Thu, 9 Mar 2000 09:27:36 -0800
> > Reply-to:      Confocal Microscopy List <CONFOCAL@listserv.acsu.buffalo.edu>
> > From:          Michael Cammer <cammer@AECOM.YU.EDU>
> > Subject:       Re: another ref. index question
> > To:            CONFOCAL@listserv.acsu.buffalo.edu
>
> > Chemists and biochemists use this technique routinely.  Also, crossed
> > polarization of transmitted light.
> >
> > At 10:03 AM 3/9/2000 -0400, you wrote:
> > >Yes, for years  oceanographers have been using refractometers to
> > >measure salinity of seawater; e.g. oceanic water at salinity 30 ppm has
> > >the refractive index of 1.025 (distilled water RI=1.000). I have never
> > >measured buffers, but if you could  get hold of a refractometer, you can
> > >do a measurement  in a second.
> >
> > *********************************************************************
> > *  Michael Cammer * Analytical Imaging Facility * Albert Einstein   *
> > *  College of Medicine * 1300 Morris Park Ave. * Bronx, NY  10461   *
> > *  (718) 430-2890  *  URL: http://www.aecom.yu.edu/aif/welcome.htm  *
> > *  personal URL: http://cammer.home.mindspring.com/index.htm        *
> > *********************************************************************
> >
>
> Colin S. Izzard, Ph.D.
> Department of Biological Sciences.
> State University of New York,
> 1400 Washington Avenue,
> Albany, N.Y. 12222, USA.
> Tel. (518)442-4367.  Fax. (518)442-4767.
> e-mail csizzard@csc.albany.edu