Nature Methods article: YFP>CFP photoconversion

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#65

From: Stephan Lange
Date: Tue, 08 Nov 2005 21:56

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Hello,

  anyone came accross the phenomenon that YFP emission spectra change 
into CFP-like emission spectra upon photobleaching?

see "Photoconversion of YFP into a CFP-like species during acceptor 
photobleaching FRET experiments"Guillaume Valentin, C�line Verheggen, 
Tristan Piolot, Henry Neel, Ma�t� Coppey-Moisan & Edouard Bertrand 
doi:10.1038/nmeth1105-801; 
http://www.nature.com/nmeth/journal/v2/n11/index.html)

A friend of mine tried it today with pEYFP (Clontech) in tranfected and 
PFA fixed Cos-1 cells and could not reproduce the reported effect...?!
Any help or experience in that case would be highly appreciated...

kind Regards
Stephan

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