Re: Nature Methods article: YFP>CFP photoconversion

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From: rene
Date: Thu, 10 Nov 2005 15:18

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Couldn't reach the article, nor do I know ins and outs of fret, but 2
things come to mind: if yfp changes to cfp, this would imply changes on
molecular level, therefore 1) is fixation allowed, and 2) would it not
be important to use exactly the right yfp??

Just a thought, Rene.


Stephan Lange schreef:

> Hello,
>
>   anyone came accross the phenomenon that YFP emission spectra change
> into CFP-like emission spectra upon photobleaching?
>
> see "Photoconversion of YFP into a CFP-like species during acceptor
> photobleaching FRET experiments"Guillaume Valentin, Céline Verheggen,
> Tristan Piolot, Henry Neel, Maïté Coppey-Moisan & Edouard Bertrand
> doi:10.1038/nmeth1105-801;
> http://www.nature.com/nmeth/journal/v2/n11/index.html)
>
> A friend of mine tried it today with pEYFP (Clontech) in tranfected and
> PFA fixed Cos-1 cells and could not reproduce the reported effect...?!
> Any help or experience in that case would be highly appreciated...
> 
> kind Regards
> Stephan

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From: "rene" <renevanwezel@hotmail.com>
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Subject: Re: Nature Methods article: YFP>CFP photoconversion
Date: 10 Nov 2005 07:18:37 -0800
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