Re: Nature Methods article: YFP>CFP photoconversion

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#67

From: Stephan Lange
Date: Thu, 10 Nov 2005 16:22

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Yeah, Nature requires that your institution has a special subscription 
to view the new arcticles... (no comment about that; grrrr)

fixation is allowed. They have shown the effect on fixed cells as well 
as YFP coupled to beads (non fixed)

They used also different types of YFP. The one from Clontech as well as 
other YFP variants (see supplementary data). All investigated YFP-types 
showed this "phenomenon"...

rene wrote:

> Couldn't reach the article, nor do I know ins and outs of fret, but 2
> things come to mind: if yfp changes to cfp, this would imply changes on
> molecular level, therefore 1) is fixation allowed, and 2) would it not
> be important to use exactly the right yfp??
> 
> Just a thought, Rene.
> 
>

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Date: Thu, 10 Nov 2005 16:22:08 +0000
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