Re: Double label florescence

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#7734

From: Natalie Bilyk
Date: Sun, 28 May 1995 00:00

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> Texas Red and FITC works well for us.
>
> > I was wondering what you folks thought produed the best double label
> > florescence for colocalization studies.  I tried TRITC and FITC and
> > thought we had a little bleeding through.  Any other suggestions ???
>

I would second that combination.  We work with FITC and Texas Red and
usually get very good results.  One occaisional problem though - when
scanning on dual green/red mode I sometimes come across a background
staining that is excited by blue light but emits in the texas red
wavelength.  This forces me to abandon dual scanning and scan only for
green or red emmission separately, which isn't always desirable.  I'm
curious to know if others have seen the same problem ?

Natalia Bilyk
Department of Anatomy and Human Biology
University of Western Australia

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From: Natalie Bilyk <nbilyk@UNIWA.UWA.EDU.AU>
Subject: Re: Double label florescence
Date: 1995/05/28
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