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Article #7738double label fluorescence
From: Jeff Craig
Date: Mon, 29 May 1995 00:00
Date: Mon, 29 May 1995 00:00
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1962 bytes
On the subject of dual label fluorescence, I have performed a little comparison of absorbtion and emission spectra from various fluorochromes and worked out which 2 would be compatible for the microscopes/filters we have. I copied the spectra from the following molecules onto transparencies and overlayed combinations together with the absorbtion/emission details of each microscope. For our BioRad MRC 600 confocal, which excites at 514nm and emits at 525-555nm and >600nm, the following combinations would theoretically have no bleed-through: FITC and TexasRed (or Propidium Iodide DNA stain) FluorX and TexasRed (or BOPRO3 or YOYO3 DNA stains) TRITC and YOYO3 DNA stain CY3 & CY5: no match although I didn't test BoDIPY for compatibility. These are only theoretical, but it seemed a useful way of screening possible cmpatible fluorochromes p.s I accidentally deleted the references quoted on this subject: could whoever posted them post me a copy, please? Jeff ********************************************************************** Jeff Craig __ Chromosome Biologist ( ) Medical Research Council Human Genetics Unit, Edinburgh, UK () jeff@hgu.mrc.ac.uk ( ) tel. 44(0)131-332-2471 ( ) fax. 44(0)131-343-2620 (__) **********************************************************************
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 114d69,e64ea59b26fc3fb1,start X-Google-Attributes: gid114d69,public From: Jeff Craig <jeff@HGU.MRC.AC.UK> Subject: double label fluorescence Date: 1995/05/29 Message-ID: <QFC9A553@kiri> X-Deja-AN: 103430296 sender: Confocal Microscopy List <CONFOCAL@UBVM.CC.BUFFALO.EDU> comments: Gated by NETNEWS@AUVM.AMERICAN.EDU newsgroups: bit.listserv.confocal