Re: Double label florescence

Article View: bit.listserv.confocal

Article #7737

Back to bit.listserv.confocal Previous Next

#7737

From: Erik Manders
Date: Mon, 29 May 1995 00:00

38 lines
2273 bytes

> I was wondering what you folks thought produed the best double label
> florescence for colocalization studies.  I tried TRITC and FITC and
> thought we had a little bleeding through.  Any other suggestions ???

The combination FITC - Texas Red is OK. You probably
will have to use an other emission filter for the red channel.

We are building a set-up for the simultaneous detection of three
different colours (FITC-TexasRed-Cy5) almost without any crosstalk.
This method is based on the modulation of the excitation beams and
demodulation of the detected signals.


Read about fluorophore choice:
Brelje, Wessendorf, Sorenson (1993) Multicolour laser scanning confocal
immunofluorescence microscopy: Practical applications and limitations.
Methods in cell biology vol. 38 p97-181

Read about intensity modulated beam scanning (IMS):
Carlsson, Aslund, Mossberg, Philip (1994) Simultaneous confocal recording
of multiple fluorescent labels with improved channel separation.
J. Microscopy 176: 286-299
Manders, Patwardhan, Carlsson (1995) Multi-channel confocal microscopy
by means of intensity-modulated multiple-beam scanning (IMS).
Zoological studies 34 supp I, p189

Erik Manders

____________________________________________________________________________

            Erik Manders                |
             Physics IV                 |       Phone:  +46-8-7906216
    Royal Institute of Technology       |       Fax:    +46-8-205609
         S-100 44 Stockholm             |       E-mail: erik@fysik4.kth.se
              Sweden                    |       Web: http://www.fysik4.kth.se

_____________________________________________________________________________
                              _____________________

Technical Details

Message-ID: <CONFOCAL%95052904544831@UBVM.CC.BUFFALO.EDU>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 114d69,907ce7b26a228ae2
X-Google-Attributes: gid114d69,public
From: Erik Manders <erik@FYSIK4.KTH.SE>
Subject: Re: Double label florescence
Date: 1995/05/29
Message-ID: <CONFOCAL%95052904544831@UBVM.CC.BUFFALO.EDU>
X-Deja-AN: 103430293
sender: Confocal Microscopy List <CONFOCAL@UBVM.CC.BUFFALO.EDU>
              swanson on sat, 27 may 1995 12: 57:56 GMT)
comments: Gated by NETNEWS@AUVM.AMERICAN.EDU
newsgroups: bit.listserv.confocal