Fluorescence Resonance Energy Transfer (FRET)

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#7783

From: Peter B. Sargent
Date: Wed, 17 May 1995 00:00

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We are interested in examining protein-protein interactions in the plane
of the membrane using FRET and wonder if anyone out there has any
experience with this technique.  The plan is to label proteins indirectly
via a Fab or IgG to which has been added a donor or acceptor.  The
classic D/A pair seems to be FITC/TRITC, but we may try Cy3/Cy5.

Thanks

Peter Sargent
UCSF

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From: "Peter B. Sargent" <sargent@ITSA.UCSF.EDU>
Subject: Fluorescence Resonance Energy Transfer (FRET)
Date: 1995/05/17
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